Synergistic effects of Mg-substitution and particle size of chicken eggshells on hydrothermal synthesis of biphasic calcium phosphate nanocrystals  

作　　者：Wei Cui Qibin Song Huhu Su Zhiqing Yang Rui Yang Na Li Xing Zhang 

机构地区：[1]Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110016,China [2]School of Materials Science and Engineering,University of Science and Technology of China,Hefei 230026,China [3]Department of Plastic Surgery,Shengjing Hospital of China Medical University,Shenyang 110004,China

出　　处：《Journal of Materials Science & Technology》2020年第1期27-36,共10页材料科学技术（英文版）

基　　金：supported by the National Key Research and Development Program of China from Ministry of Science and Technology(2016YFC1100502);Key Research Program of Frontier Sciences(QYZDJ-SSW-JSC031)from Chinese Academy of Sciences(CAS);Key Research and Development Program of Liaoning Province(2017105005);Shenyang Key R&D and Technology Transfer Program(Z17-7-023)。

摘　　要：Magnesium(Mg^2+))ion plays important roles in biomineralization of bone,teeth and calcium carbonate skeletons.Herein,chicken eggshells mainly comprising of Mg-calcite nanocrystals(Mg/(Mg+Ca)2.0 mol.%)were used to fabricate biphasic calcium phosphate(BCP),a mixture of hydroxyapatite(HA)and p-tricalcium phosphate(p-TCP)nanocrystals,through hydrothermal reactions at 200℃for 24 h.Our results indicated thatβ-TCP nanocrystals formed through the ion-exchange reactions of Mg-calcite,while HA nanocrystals were mainly produced by dissolution-reprecipitation reactions on the surfaces of eggshell samples in the hydrothermal system.Mg substitution in calcite resulted in formation ofβ-TCP nanocrystals instead of HA crystals through ion-exchange reactions.BCP samples with different compositions(28.6-77.8 wt.%β-TCP)were produced by controlling particle sizes of eggshells for hydrothermal reactions.The larger particles lead to the larger proportion ofβ-TCP in the BCP composition.Therefore,Mg substitution and particle size had synergetic effects on the hydrothermal synthesis of BCP using chicken eggshells through balance of ion-exchange and dissolution-reprecipitation reactions.Cell culture results showed that the BCP products were non-cytotoxic to MC3 T3-E1 cells,which may be used for bone substitute materials in future.Magnesium(Mg2+) ion plays important roles in biomineralization of bone,teeth and calcium carbonate skeletons.Herein,chicken eggshells mainly comprising of Mg-calcite nanocrystals（Mg/（Mg+Ca）2.0 mol.%） were used to fabricate biphasic calcium phosphate（BCP）,a mixture of hydroxyapatite（HA）and p-tricalcium phosphate（p-TCP） nanocrystals,through hydrothermal reactions at 200℃for 24 h.Our results indicated that β-TCP nanocrystals formed through the ion-exchange reactions of Mg-calcite,while HA nanocrystals were mainly produced by dissolution-reprecipitation reactions on the surfaces of eggshell samples in the hydrothermal system.Mg substitution in calcite resulted in formation of β-TCP nanocrystals instead of HA crystals through ion-exchange reactions.BCP samples with different compositions（28.6-77.8 wt.% β-TCP） were produced by controlling particle sizes of eggshells for hydrothermal reactions.The larger particles lead to the larger proportion of β-TCP in the BCP composition.Therefore,Mg substitution and particle size had synergetic effects on the hydrothermal synthesis of BCP using chicken eggshells through balance of ion-exchange and dissolution-reprecipitation reactions.Cell culture results showed that the BCP products were non-cytotoxic to MC3 T3-E1 cells,which may be used for bone substitute materials in future.

关 键 词：HYDROTHERMAL reactions Ion-exchange Dissolution-reprecipitation NANOCRYSTALS BIPHASIC calcium phosphate Bone substitute materials 

分 类 号：TB383.1[一般工业技术—材料科学与工程]