辽诱系列玉米单倍体诱导系的选育  

Breeding of LAAS-set Haploid Inducers with High Frequency Parthenogenesis in Maize

作　　者：刘欣芳[1] 马骏[1] 齐欣[1] 弓雪[1] 孟庆国[1] Eduard Khatefov Suprunov Anatoly 赵念力[1] 白添 姜敏[1] 王延波[1] LIU Xin-fang;MA Jun;QI Xin;GONG Xue;MENG Qing-guo;Eduard Khatefov;Suprunov Anatoly;ZHAO Nian-li;BAI Tian;JIANG Min;WANG Yan-bo(Corn Research Institute,Liaoning Academy of Agricultural Science,Shenyang 110161;Federal Research Center All-Russian Scientific Research Institute of Plant Genetic Resources named after N.I.Vavilov(VIR),Russia;National Center of Grain named after P.P.Lukyanenko,Federal State Budget Scientific Organization,Russia;Northeat Yucai School,Shenyang 110179,China)

机构地区：[1]辽宁省农业科学院玉米研究所,沈阳110161 [2]俄联邦研究中心瓦维洛夫全俄植物遗传资源研究所,俄罗斯 [3]俄联邦国家预算科学机构P.P.国家谷类研究中心,俄罗斯 [4]东北育才学校,沈阳110179

出　　处：《玉米科学》2020年第1期25-30,共6页Journal of Maize Sciences

基　　金：农业部转基因生物新品种培育重大专项(2016ZX08003003-008);辽宁省农业领域青年科技创新人才培养计划项目(2015024);辽宁省科技厅“中俄(辽宁)创新创业基地项目”

摘　　要：采用多个测验种逐代跟踪测定诱导率的方法,利用俄罗斯引进的单倍体诱导系资源,经过5年7代选育出辽诱系列玉米单倍体诱导系辽诱1号、辽诱2号、辽诱3号和辽诱4号。对22个品种的诱导效果试验表明,辽诱1号、辽诱2号、辽诱3号和辽诱4号的综合诱导率分别为6.85%、7.53%、6.66%和6.03%,比对照高诱五号分别高出0.6倍、0.8倍、0.6倍和0.4倍。诱导基因检测结果表明,辽诱系列诱导系具有诱导率高、花粉量大、抗病、抗倒、易繁殖等优良特性,且存在和高诱五号共同的诱导基因GRMZM2G471240,是开展玉米单倍体育种的理想诱导系。A batch of haploid inducer resources was introduced from Russia in 2013 and used for breeding.LAAS-1,LAAS-2,LAAS-3 and LAAS-4 were bred by using multi-test materials with high frequency inducing rate(IR)through 7 generations between 5 years.The results of induction effect test for 22 entries showed that the IR of LAAS-1,LAAS-2,LAAS-3 and LAAS-4 were 6.85%(0.6 times higher than CK),7.53%(0.8 times higher than CK),6.66%(0.6 times higher than CK)and 6.03%(0.4 times higher than CK),respectively.The results of induced gene test indicated that the LAAS-set haploid inducers and CAU-5 shared the same induced gene GRMZM2 G471240.LAAS-set haploid inducers are ideal inducers for haploid breeding of maize at present in good characteristics such as high inducing rate,abundant pollen quantity,resistance to blight and lodging,easy reproduction,etc.

关 键 词：玉米 单倍体诱导系 平均诱导率 综合诱导率 诱导基因 

分 类 号：S513.035.3[农业科学—作物学]