玉米株高qPH10的QTL定位及候选基因预测  

QTL Mapping and Candidate Gene Prediction of Maize Plant Height qPH10

作　　者：卢晓环 周志强[1] 李海灵 李冬冬[2] 刘文欣[2] 李明顺[1] LU Xiao-huan;ZHOU Zhi-qiang;LI Hai-ling;LI Dong-dong;LIU Wen-xin;LI Ming-shun(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]中国农业大学,北京100193

出　　处：《玉米科学》2020年第1期37-44,共8页Journal of Maize Sciences

摘　　要：利用掖478为轮回亲本、齐319为供体亲本构建的染色体片段代换系CL137为父本,与掖478杂交构建近等基因系F2分离群体,根据齐319、掖478重测序数据开发在双亲中具有多态性的Indel分子标记,在两个环境中对控制玉米株高的10号染色体QTL进行定位。结果表明,2017年的株高表型将QTL定位到标记mk8-bnlg1655之间,位于83.86~85.34 Mb(B73 RefGen_v3)的1.5 Mb区间,表型贡献率为7.57%;2018年株高表型将株高QTL定位到标记mk5-bnlg1655之间,位于82.76~85.34 Mb的2.5 Mb区间,表型贡献率为5.75%。同时检测发现,该QTL主要以加性效应为主,显性效应较小。通过对所定位的QTL重合区间内的基因进行功能注释,预测可能控制株高的候选基因,为后续精细定位第10号染色体株高QTL以及探索候选基因功能机制提供研究基础。A chromosomal fragment substitution line(CSSL) CL137, which was constructed with Ye478 as the recurrent parent and Qi319 as the donor parent was used as the male parent to hybridize with Ye478 to construct the F2 segregating population. The polymorphic Indel markers were developed based on the re-sequencing data of Qi319 and Ye478. The plant height QTL on chromosome 10 was identified in two environments. In 2017, the QTL of PH was mapped between the markers mk7-bnlg1655, corresponding to the 83.86-85.34 Mb interval of the B73 genome(RefGen_v3), the physical distance was about 1.5 Mb, and the phenotypic variation explanation(PVE) was7.57%. In 2018, the QTL of PH was mapped between the markers mk5-bnlg1655, corresponding to the 82.76-85.34 Mb interval of the B73 genome, the physical distance was about 2.5 Mb, and the PVE was 5.75%. Notably,the genetic effects of qPH10 were mainly additive effects. This study also annotated the functional genes in the position interval and predicted candidate genes that may control plant height. The results of this study can provide a basis for fine mapping the plant height QTL on chromosome 10 and explore the functional mechanisms of candidate genes.

关 键 词：玉米 染色体片段代换系 株高 数量性状位点 

分 类 号：S513.035.3[农业科学—作物学]