基于iTRAQ技术的干旱胁迫下玉米苗期蛋白质组学研究  

iTRAQ-based Quantitative Proteomic Analysis of Maize Seedlings in Response to Drought Stress

作　　者：姜志磊[1] 金峰学[1] 李毅丹[1] JIANG Zhi-lei;JIN Feng-xue;LI Yi-dan(Agro-Biotechnology Research Institute,Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology,Changchun 130033,China)

机构地区：[1]吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室

出　　处：《玉米科学》2020年第1期51-58,共8页Journal of Maize Sciences

基　　金：吉林省农业科技创新工程项目“ZmGA2ox6基因的深入发掘及其多效性研究”(CXGC2018ZY025);吉林省科技发展计划国际科技合作项目“ZmGA2ox类赤霉素合成调控基因在玉米耐旱响应中的研究及应用”(20160414041GH)

摘　　要：采用同位素标记相对定量(iTRAQ)技术,对干旱胁迫条件下苗期玉米的蛋白质组学变化进行分析。结果表明,共检测到玉米幼苗中的207个蛋白在干旱胁迫后发生了显著的丰度变化。根据蛋白注释情况可将这些蛋白归入信号传导、渗透调节、蛋白合成与折叠、ROS清除、膜运输、转录相关、细胞结构与细胞周期、脂肪酸代谢、碳水化合物与能量代谢、光合作用与光呼吸等代谢途径。干旱胁迫后,涉及光反应和呼气作用的差异蛋白多表现为丰度上升;涉及碳水化合物及蛋白质合成差异蛋白多表现为丰度下降;与渗透调节相关的脱水蛋白、脯氨酸代谢和渗透胁迫相关的蛋白酶则显示为丰度上升。干旱胁迫还能导致植物体内活性氧大量产生,活性氧清除相关的酶类也会发生明显的丰度上升。根据研究结果推测,玉米苗期主要通过降低植株生长速率、减少水分散失、清除自由基等多种方式维持其在干旱胁迫条件下的生长发育过程。To investigate the molecular mechanisms of drought tolerance response in maize seedlings, protein abundance changes were studied by using isotopic tagging relative quantitation(iTRAQ). A total of 207 proteins were detected to be significantly differently expressed during drought tolerance in maize seedlings. These differential proteins were divided into 10 categories including signaling, osmoregulation, protein synthesis and folding, ROS clearance, membrane transport, transcription related, cell structure and cell cycle, fatty acid metabolism, carbohydrate and energy metabolism, as well as photosynthesis and photorespiration. Most proteins involved in photoreaction and exhalation were accumulated;most of carbohydrate and protein synthesis related proteins were reduced;and proteins associated with the osmotic adjustments such as dehydrins, proline, and osmotic stress were accumulated. In addition, drought stress always induces excessive reactive oxygen species(ROS) in plants, and the significant accumulation of some ROS scavenging related enzymes was detected in this study. Comprehensive analysis of the results, maize seedlings might maintain the growth and development process under drought stress conditions by decreasing plant growth rate, reducing water loss and scavenging free radicals.

关 键 词：玉米 ITRAQ 蛋白质组学 胁迫相关蛋白 蛋白功能 

分 类 号：S513.035.3[农业科学—作物学]