转Cry1Ab-Ma基因玉米CM8101对亚洲玉米螟抗性研究  

Efficacy Evaluation of Transgenic Cry1Ab-Ma Maize CM8101 for Resistance to the Ostrinia furnacalis

作　　者：张爽 鲁鑫 张嘉月 杨小艳[3] 谢树章[3] 武凤慈 宋新元[4] 王振华[1] 李新海[1,2] 翁建峰[2] ZHANG Shuang;LU Xin;ZHANG Jia-yue;YANG Xiao-yan;XIE Shu-zhang;WU Feng-ci;SONG Xin-yuan;WANG Zhen-hua;LI Xin-hai;WENG Jian-feng(Agricultural College,Northeast Agricultural University,Harbin 150030;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;Chongqing Academy of Agricultural Sciences,Chongqing 401329;Jilin Academy of Agricultural Sciences,Changchun 130033,China)

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]中国农业科学院作物科学研究所,北京100081 [3]重庆市农业科学院,重庆401329 [4]吉林省农业科学院,长春130033

出　　处：《玉米科学》2020年第1期59-64,共6页Journal of Maize Sciences

基　　金：转基因新品种培育科技重大专项(2018ZX0800201B)

摘　　要：利用q RT-PCR和ELISA等方法测定转Cry1Ab-Ma基因玉米CM8101不同世代、不同组织目标基因表达量,开展玉米螟室内和田间生测鉴定,评价转Cry1Ab-Ma基因玉米CM8101对亚洲玉米螟的抗性。结果表明,Cry1Ab-Ma基因在CM8101各组织中均有表达,不同世代间目的基因的表达量无显著性差异,遗传稳定。室内生测结果表明,转基因玉米CM8101各组织均对亚洲玉米螟具有显著的杀虫效果,不同世代间玉米螟的存活率均无显著性差异。田间接虫鉴定结果表明,CM8101在T3、T4、T5世代的抗性等级均为1级,抗性水平为高抗。q RT-PCR and ELISA methods were used to detect the Cry1 Ab-Ma expression, and lab and field bioassay were applied to evaluate the insect resistance of transgenic Cry1 Ab-Ma maize CM8101. The Cry1 Ab-Ma genes could be detected to express in all tested tissues in CM8101, and there was no significant difference among different generations. Compared with non-transgenic maize Zheng 58, CM8101 showed significant insect resistance to Ostrinia furnacalis in different tissues both in laboratory and field conditions. And no significant differences in the survival rate of the Ostrinia furnacalis were observed in these tissues among different generations. The resistance rate of CM8101 was detected to be Class 1 at each generation, showing high resistance level to Ostrinia furnacalis.

关 键 词：转基因玉米 时空表达 亚洲玉米螟 抗虫性鉴定 

分 类 号：S513.037[农业科学—作物学]