洋参御糖丸对糖尿病大鼠心肌微血管病变的干预作用  

Effects of Yangshen Yutang Pill on Myocardial Microvascular Disease in Diabetic Rats

作　　者：隋艳波[1] 孙艺 王瑞楠 谢宁[1] SUI Yanbo;SUN Yi;WANG Ruinan;XIE Ning(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学附属第一医院,哈尔滨150040 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《吉林中医药》2020年第2期222-226,共5页Jilin Journal of Chinese Medicine

基　　金：黑龙江省教育厅科学基金面上项目(12531612)。

摘　　要：目的观察洋参御糖丸对糖尿病大鼠心肌微血管病变的干预作用。方法高糖高脂联合腹腔注射链脲佐菌素建立糖尿病大鼠心肌微血管损伤模型,筛选成模大鼠并将其随机分为模型组、二甲双胍组以及洋参御糖丸高、低剂量组,各组大鼠相应给予4周蒸馏水、二甲双胍70 mg/(kg·d)、洋参御糖丸2.7 g/(kg·d)、洋参御糖丸1.35 g/(kg·d)干预后,检测血糖、糖化血红蛋白,观察大鼠心肌超微结构以及对大鼠心肌AGEs、VEGF、VCAM-1的影响。结果洋参御糖丸高剂量组、二甲双胍组与模型组相比血糖、HbA1c均显著下降;心肌AGEs、VEGF、VCAM-1表达明显降低;电镜下心肌纤维排列整齐,肌丝结构正常,细胞核线粒体结构清晰,心肌微血管数目正常。结论洋参御糖丸在降糖同时能够修复心肌超微结构的损伤;并且具有降低AGEs、抑制病理性微血管促新生效应、抑制促内皮细胞黏附积聚作用,进而可能延缓糖尿病微血管病变进程。Objective To observe the intervention effect of Yangshen Yutang pill on myocardial microvascular disease in diabetic rats.Methods The model of myocardial microvascular injury in diabetic rats was established by high-sugar and high-fat combined with intraperitoneal injection of streptozotocin.The model rats were randomly divided into model group,metformin group and high-low dose group of Yangshen Yutang pill.Rats in the group were given 4 weeks of distilled water,metformin 70 mg/(kg·d),Yangshen Yutang pill 2.7 g/(kg·d),and Yangshen Yutang pill 1.35 g/(kg·d)to detect blood sugar and glycated hemoglobin.Ultrastructure of rat myocardium and its effects on myocardial AGEs,VEGF and VCAM-1 in rats.Results Compared with the model group,the blood glucose and HbA1 C of the high-dose group and the metformin group of the ginseng powder were significantly decreased.The expression of AGEs,VEGF and VCAM-1 was significantly decreased.The myocardial fibers were arranged neatly under the electron microscope,the myofilament structure was normal,and the mitochondria were normal.The structure is clear and the number of myocardial microvessels is normal.Conclusion Yangshen Yutang pill can repair the damage of myocardial ultrastructure while reducing blood sugar,and it can reduce AGEs,inhibit the pathogenic microvascular stimulating effect,inhibit the adhesion of endothelial cells,and possibly delay the progression of diabetic microangiopathy.

关 键 词：洋参御糖丸 糖尿病 心肌微血管病变 大鼠 

分 类 号：R285.5[医药卫生—中药学]