球孢白僵菌催化合成R-(+)-2-(4-羟基苯氧基)丙酸的菌种选育  

Breeding of Strains for the Synthesis of R-(+)-2-(4-Hydroxyphenoxy)Propionic Acid by Beauveria bassiana

作　　者：郝会会 王灿 张倩[1] 赵筱[1] 王金华[1] HAO Hui-hui;WANG Can;ZHANG Qian;ZHAO Xiao;WANG Jin-hua(Key Laboratory of Fermentation Engineering of Ministry of Education,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学发酵工程教育部重点实验室,武汉430068

出　　处：《农药》2020年第2期94-98,102,共6页Agrochemicals

基　　金：国家“十二五”支撑计划(2012BAD27B03);湖北省自然科学基金项目(2015CFB637);湖北工业大学博士科研启动基金项目(BSQD12143)。

摘　　要：[目的]为采用生物转化法合成R-(+)-2-(4-羟基苯氧基)丙酸(D-HPPA)提供优良菌株及理论基础。[方法]针对球孢白僵菌,先采用紫外(UV)诱变筛选高耐受R-(+)-2-苯氧基丙酸(D-PPA)的突变株,再使用等离子体(ARTP)诱变、N-甲基-N′-硝基-N-亚硝基胍(MNNG)诱变及ARTP和MNNG复合诱变提高突变株底物转化率。[结果]得到突变株UA1052,其底物转化率为98.5%、平均每天产物积累速率为6.93 g/(L·d)(较出发菌提高了796.6%),且遗传性稳定。[结论]ARTP诱变可大幅提高球孢白僵菌催化合成D-HPPA的能力。[Aims]This research aims to provide an excellent strain and theoretical basis for the production of R-(+)-2-(4-hydroxyphenoxy)propionic acid(D-HPPA)by biotransformation.[Methods]The mutant strain of Beauveria bassiana with high tolerance to R-(+)-2-phenoxypropionic acid(D-PPA)was generated by ultraviolet(UV)mutagenesis firstly.Then the plasma was further induced by ARTP Mutagenesis,N-methyl-N-nitro-N-nitrosoguanidine(MNNG)mutagenesis and ARTP and MNNG complex mutagenesis separately to enhance the ability of transform D-PPA into D-HPPA.[Results]The mutant strain UA1052 was obtained with a substrate conversion rate of 98.5%and an average daily product accumulation rate of 6.93 g/(L-d)(up to 796.6%times that starting bacteria),with stable heritability.[Conclusions]The ability to catalyze the synthesis of D-HPPA by B.bassiana can be greatly improved by ARTP mutagenesis.

关 键 词：球孢白僵菌 生物催化 R-(+)-2-苯氧基丙酸 R-(+)-2-(4-羟基苯氧基)丙酸 菌种诱变 

分 类 号：TQ450[化学工程—农药化工]