猪繁殖与呼吸综合征病毒山西分离株ORF7基因序列分析  

Sequence Analysis on the ORF7 Gene of Shanxi Isolate Porcine Reproductive and Respiratory Syndrome Virus

作　　者：樊振华[1] 刘文俊[1] 武守艳[1] 吴忻[1] 孟帆[1] 焦福林[1] 薛翼鹏[1] FAN Zhenhua;LIU Wenjun;WU Shouyan;WU Xin;MENG Fan;JIAO Fulin;XUE Yipeng(Institute of Animal Husbandry and Veterinary,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China)

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032

出　　处：《山西农业科学》2020年第3期446-448,共3页Journal of Shanxi Agricultural Sciences

基　　金：山西省农业科学院农业科技创新研究课题(YCX2020105,YCX2018D2YX08);山西省重点研发计划(指南)项目(201703D221023-2);山西省农业科学院特色农业技术攻关项目(YGG17089)。

摘　　要：采用RT-PCR方法对疑似猪繁殖与呼吸综合征(PRRS)阳性病料进行克隆和测序,获得2个ORF7基因片段,并对其基因序列和推导的氨基酸序列与国内外毒株进行了同源性比较分析。序列分析结果显示,2个ORF7基因之间核苷酸和氨基酸同源性分别为99.7%和98.4%,其与欧洲型代表毒株LV、美洲型代表毒株VR-2332、高致病性PRRSV变异株(JXA1、HUN4、HEB1、HUB1)及我国早期分离毒株CH-1a的核苷酸同源性分别为65.9%、93.0%~93.3%、97.6%~98.1%、94.6%~94.9%,氨基酸同源性分别为58.1%、93.5%~94.4%、95.2%~96.0%、91.9%~92.7%。遗传进化树分析结果表明,分离的2个ORF7基因与美洲型代表毒株VR-2332亲缘关系较近,属于美洲型,与CH-1a、HB-2(sh)、我国2006年以后分离的10株(JXA1、SD-09、HUB1、HEB1、HUN4、Henan-A14、GD、GDQY2、GZgy15-1、GD-2011)组成同一个亚群。Through RT-PCR amplification of tissue or blood sample from Shanxi province,the 2 ORF7 gene nucleotide sequences were obtained.The obtained sequences and the deduced amino acid were analyzed and compared with the other published PRRSV srains.Sequence analysis results showed that the nucleotide and amino acid homology were 99.7%and 98.4%,respectirely,among 2 ORF7,comparing with LV,VR-2332,highly pathogenic PRRSV(JXA1,HUN4,HEB1,HUB1),CH-1a,the nucleotide homology were 65.9%,93.0%-93.3%,97.6%-98.1%,94.6%-94.9%,respectirely.The AA homology were 58.1%,93.5%-94.4%,95.2%-96.0%,91.9%-92.7%,respectively.The results of phylogenetic tree analysis showed that the prevalent PRRSV strains in Shanxi belong to north American genotopy which formed the same subgroup with CH-1a,HB-2(sh),isolated 10 strains after 2006.

关 键 词：猪繁殖和呼吸综合征病毒 ORF7基因 序列分析 

分 类 号：S858.28[农业科学—临床兽医学]