Ⅲ~Ⅳ期糖尿病肾病不同中医证型的血清蛋白组学研究  

Serum proteomics of different traditional Chinese medicine syndrome types of stage Ⅲ-Ⅳ diabetic nephropathy

作　　者：刘垠浩 陈丽贞[1] LIU Yinhao;CHEN Lizhen(Zhangzhou Hospital of Traditional Chinese Medicine Affiliated to Fujian University of Traditional Chinese Medicine,Zhangzhou 363000,Fujian,China)

机构地区：[1]福建中医药大学附属漳州市中医院,福建漳州363000

出　　处：《湖南中医杂志》2020年第1期7-10,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：福建省卫生健康委员会青年科研课题(2016-2-42)。

摘　　要：目的:探索Ⅲ~Ⅳ期糖尿病肾病(DN)患者血清差异蛋白,同时筛选不同中医证型的血清差异蛋白。方法:收集Ⅲ~Ⅳ期DN患者70例(气阴两虚证19例,脾肾气虚证18例,血瘀证16例,湿热证17例),同时选择健康受试者35例(健康对照组),应用表面加强激光解吸电离-飞行时间-质谱(SELDI-TOF-MS)技术检测各组血清蛋白指纹图谱,并对差异蛋白峰进行对比分析。结果:1) DN组与健康对照组之间共有9个蛋白峰存在显著差异,质荷比(M/Z)分别为2042.57、3291.28、4986.15、5312.69、5564.09、9861.47、10786.53、13392.89、17395.27;2)气阴两虚证与脾肾气虚证之间共有4个蛋白峰存在显著差异,M/Z分别为2994.77、4986.15、7937.25、2758.91;3)气阴两虚证与血瘀证之间共有4个蛋白峰存在显著差异,M/Z分别为4986.15、16982.62、6819.69、9947.36;4)气阴两虚证与湿热证之间共有3个蛋白峰存在显著差异,M/Z分别为4986.15、11741.33、7001.54;5)脾肾气虚证与血瘀证之间共有3个蛋白峰存在显著差异,M/Z分别为3448.22、8063.43、9787.21;6)脾肾气虚证与湿热证之间共有5个蛋白峰存在显著差异,M/Z分别为2144.34、3992.01、8871.35、10568.32、14643.47;7)血瘀证与湿热证之间共有6个蛋白峰存在显著差异,M/Z分别为4233.15、5771.32、5987.18、8496.76、6651.25、13551.94。结论:Ⅲ~Ⅳ期DN组与健康对照组血清蛋白表达存在差异,该差异或可作为相关临床诊断的标志物,对探索DN的发病机制具有重要的参考价值;在不同中医证型之间能够找到差异血清蛋白,可能反映"证"的实质内涵,对今后建立诊断决策模型具有积极意义。Objective: To investigate the differentially expressed proteins in the serum of patients with stage Ⅲ-Ⅳ diabetic nephropathy(DN) or those with different syndrome types.Methods: A total of 70 patients with stage Ⅲ-Ⅳ DN were enrolled as DN group,among whom 19 had deficiency of both Qi and Yin,18 had spleen-kidney Qi deficiency,16 had blood stasis syndrome,and 17 had damp-heat syndrome,and 35 healthy individuals were enrolled as healthy control group.Surface-enhanced laser desorption/ionization-time of flight-mass spectrometry was used to obtain the fingerprints of serum proteins,and the peaks of differentially expressed proteins were analyzed.Results: There were significant differences in 9 protein peaks between the DN group and the healthy control group,with a mass-to-charge ratio(M/Z) of 2042.57,3291.28,4986.15,5312.69,5564.09,9861.47,10786.53,13392.89,and 17395.27,respectively.There were significant differences in 4 protein peaks between the patients with deficiency of both Qi and Yin and those with spleen-kidney Qi deficiency,with an M/Z of 2994.77,4986.15,7937.25,and 2758.91,respectively.There were significant differences in 4 protein peaks between the patients with deficiency of both Qi and Yin and those with blood stasis syndrome,with an M/Z of 4986.15,16982.62,6819.69,and 9947.36,respectively.There were significant differences in 3 protein peaks between the patients with deficiency of both Qi and Yin and those with damp-heat syndrome,with an M/Z of 4986.15,11741.33,and7001.54,respectively.There were significant differences in 3 protein peaks between the patients with spleen-kidney Qi deficiency and those with blood stasis syndrome,with an M/Z of 3448.22,8063.43,and 9787.21,respectively.There were significant differences in 5 protein peaks between the patients with spleen-kidney Qi deficiency and those with damp-heat syndrome,with an M/Z of 2144.34,3992.01,8871.35,10568.32,and 14643.47,respectively.There were significant differences in 6 protein peaks between the patients with blood stasis syndrome a

关 键 词：糖尿病肾病 中医证型 血清蛋白组学 表面加强激光解吸电离-飞行时间-质谱技术 

分 类 号：R259.872[医药卫生—中西医结合]