β-环糊精修饰Fe3O4纳米粒用于药物递送系统的研究  

Study on β-cyclodextrin modified Fe3O4 nanoparticles as drug delivery system

作　　者：高金龙[1,2] 龚敬捧 刘永明 李宛萍 王奎涛 GAO Jin-long;GONG Jing-peng;LIU Yong-ming;LI Wan-ping;WANG Kui-tao(School of Chemical and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China;Hebei Provincial Key Laboratory of Molecular Chemistry for Drug,Shijiazhuang 050018,China;Shijiazhuang Pharmaceutical Group Co.,Ltd.,Shijiazhuang 050035,China)

机构地区：[1]河北科技大学化学与制药工程学院,河北石家庄050018 [2]河北省药用分子化学重点实验室,河北石家庄050018 [3]石药集团,河北石家庄050035

出　　处：《现代化工》2020年第2期157-161,共5页Modern Chemical Industry

基　　金：河北省自然科学基金(B2016208054)

摘　　要：利用层层组装法合成了羧甲基-β-环糊精修饰的Fe3O4纳米粒,以疏水型药物左旋咪唑(LMS)为模型药物,制备了载药纳米粒并对其细胞毒性和体外释药特性进行考察。利用IR、XRD、TEM、Zeta电位与激光粒度仪等对材料进行结构形貌表征,利用UV法测定载药纳米粒的载药量和包封率并进行体外释放药物左旋咪唑性能研究,采用MTT法评价材料的细胞毒性。结果表明,羧甲基-β-环糊精成功地接枝到四氧化三铁纳米粒上,粒径大小为22. 4 nm左右,呈球状或椭圆状,磁性良好。当载体与药摩尔比为1∶1时,对左旋咪唑的载药量和包封率分别为(14. 32±0. 24)%和(42. 38±0. 35)%,复合材料对MCF-7细胞无毒,载药纳米粒在缓冲溶液中具有较好的缓释效果。Carboxymethyl-β-cyclodextrin modified Fe3O4 nanoparticles are synthesized through a layer-by-layer assembly method.Levamisole( LMS),a hydrophobic drug,is used as model drug to prepare drug-loading nanoparticles,and their cytotoxicity and in vitro drug release characteristics are investigated. The structure and morphology of the nanoparticles are characterized by IR,XRD,TEM,Zeta potential and laser particle size analyzer.The loading amount and encapsulation rate of the nanoparticles to drug are determined by UV method. The in vitro release properties of the nanoparticles for levamisole are studied,and the cytotoxicity is evaluated by MTT method. Results show that carboxymethyl-β-cyclodextrin has successfully been grafted onto Fe3O4 nanoparticles.The prepared nanoparticles have an average particle size of about 22. 4 nm,an isoelectric point of 5. 3 and a dispersion coefficient less than 0. 2.They exhibit spherical or elliptical profile and show good magnetic properties. The loading amount and encapsulation rates of the nanoparticles for levamisole can reach( 14. 32 ± 0. 24) % and( 42. 38 ± 0. 35) %,respectively when the molar ratio of nanoparticles to drug is 1 ∶ 1. This kind of nanoparticles have no toxicity to MCF-7 cells,and the drug-loading nanoparticles have good slow release effect in buffer solution.

关 键 词：环糊精 左旋咪唑(LMS) 药物递送 细胞毒性 缓释 

分 类 号：R944[医药卫生—药剂学]