虎红探针测定盐酸美西律——基于双波长共振光散射技术  

Determination of mexiletine hydrochloride by rose Bengal probe using dual-wavelength resonance light scattering technology

作　　者：江虹[1] 庞向东[1] 胡影 JIANG Hong;PANG Xiang-dong;HU Ying(College of Chemistry and Chemical Engineering,Collaborative Innovation Center for Green Development in Wuling Mountain Areas,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院化学化工学院武陵山片区绿色发展协同创新中心

出　　处：《药学学报》2020年第2期298-304,共7页Acta Pharmaceutica Sinica

基　　金：重庆市教委科技基金资助项目(KJ1401226);长江师范学院科技基金资助项目(2018CXX117)

摘　　要：探讨了虎红与盐酸美西律在溴代十六烷基吡啶存在下相互作用的共振光散射(RLS)光谱特征,建立了测定药物中盐酸美西律的双波长共振光散射(DWO-RLS)新方法。在弱酸性溶液中,虎红与盐酸美西律及溴代十六烷基吡啶相互作用生成红色三元缔合物,使共振光散射信号显著增强,并在372和596 nm波长处产生2个强的特征散射峰。在这二波长处,盐酸美西律在0.004~0.65 mg·L^-1内的质量浓度与共振光散射增强强度(ΔIRLS)呈线性关系,检出限分别为0.0032 mg·L^-1(372 nm)和0.0038 mg·L^-1(596 nm)。当用DWO-RLS技术测定时,其检出限更低,仅为0.0018 mg·L^-1,将DWO-RLS法用于市售盐酸美西律药片中盐酸美西律的测定,回收率为98.5%~103%,相对标准偏差为2.0%~2.7%。The resonance light scattering(RLS)spectral characteristics of the interaction between rose Bengal and mexiletine hydrochloride in the presence of cetylpyridinium bromide were investigated.A dual-wavelength resonance light scattering(DWO-RLS)method for the determination of mexiletine hydrochloride in drugs was established.In a weakly acidic solution,rose Bengal interacts with mexiletine hydrochloride and cetylpyridinium bromide to form a red ternary ion association complex,which led to a significantly enhanced resonance light scattering signal and produced two strong characteristic scattering peaks at 372 nm and 596 nm.In these two wavelengths the mass concentration of mexiletine hydrochloride was in the range of 0.004 to 0.65 mg·L^-1 and had a good linear relationship with the resonance light scattering enhancement intensity(ΔIRLS),with detection limits of 0.0032 mg·L^-1(372 nm)and 0.0038 mg·L^-1(596 nm),respectively.When measured by the dual-wavelength resonance light scattering(DWO-RLS)technique,the detection limit was lower,only 0.0018 mg·L^-1.When the DWO-RLS method was applied to the determination of mexiletine hydrochloride in commercially available mexiletine hydrochloride tablets,and the recovery was 98.5%-103%,and the relative standard deviation was 2.0%-2.7%.

关 键 词：盐酸美西律 溴代十六烷基吡啶 虎红 双波长 共振光散射 

分 类 号：R917[医药卫生—药物分析学] O657.39[医药卫生—药学]