荧光定量PCR法检测4212例生殖道沙眼衣原体标本结果分析  

Analysis on 4212 specimens of Chlamydia trachomatis detection by fluorescence quantitative PCR

作　　者：梁悦[1,2] 马会平 张强[4] 周晓丽 宋琳琳 关长吉 蔺美娜 郭廷超[1,2] 

机构地区：[1]中国医科大学国家卫健委生殖健康与遗传医学重点实验室,辽宁沈阳110031 [2]辽宁省计划生育科学研究院,辽宁沈阳110031 [3]沈阳市妇婴医院生殖中心,辽宁沈阳110011 [4]天津医科大学公共卫生学院劳动卫生与环境卫生学系,天津300070

出　　处：《中国妇幼保健》2020年第3期550-553,共4页Maternal and Child Health Care of China

基　　金：辽宁省博士启动基金(201601386)

摘　　要：目的分析荧光定量PCR (FQ-PCR)法检测4 212例生殖道沙眼衣原体(CT)标本的结果。方法采用FQ-PCR与胶体金法检测4 212例生殖道CT标本,检测阳性的标本进行核酸测序分析,分析阳性结果的真实性。结果 FQ-PCR法和胶体金法检测生殖道CT阳性检出率分别为1. 94%(82例)和0. 24%(10例),差异有统计学意义(P<0. 05)。FQ-PCR法检测CT阳性标本核酸成功测序,结果分析均为CT。结论 FQ-PCR法检测CT的检出率高、敏感、结果真实可信,是生殖健康、优生优育、不孕不育检查早期发现并预防CT感染的可信方法。Objective To analyze the detection results of Chlamydia trachomatis( CT) by fluorescence quantitative PCR( FQ-PCR).Methods FQ-PCR and colloidal gold method were used to detect 4 212 CT specimens,then the positive specimens underwent nucleic acid sequencing analysis,the authenticity of positive results was analyzed. Results The positive rates of genital tract CT detected by FQ-PCR and colloidal gold method were 1. 94%( 82 cases) and 0. 24%( 10 cases),respectively,there was statistically significant difference( P<0. 05). Nucleic acid sequencing succeeded in CT positive specimens detected by FQ-PCR,the results consisted with each other. Conclusion FQ-PCR has the advantages of high detection rate,sensitivity,true,and credibility,which is a reliable method for reproductive health,eugenics,early infection of fertility,and prevention of CT infection.

关 键 词：沙眼衣原体 荧光定量PCR 生殖健康 

分 类 号：R711.3[医药卫生—妇产科学]