一种聚合型弱阳离子交换/亲水相互作用色谱固定相的制备及色谱性能  

Preparation and chromatographic evaluation of a polymer-based stationary phase for weak anion-exchange/hydrophilic interaction

作　　者：娄旭华 左慧颖 王媛 赵文杰 LOU Xuhua;ZUO Huiying;WANG Yuan;ZHAO Wenjie(School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学化学化工学院

出　　处：《色谱》2020年第4期430-437,共8页Chinese Journal of Chromatography

基　　金：国家自然科学基金(21675040);河南省高校创新人才资助项目(20HASTIT007)~~

摘　　要：通过亲核取代反应将聚乙烯马来酸酐键合到氨基硅胶表面,然后将残余的马来酸酐水解,制备了一种弱阳离子交换/亲水相互作用高效液相色谱固定相(Sil-PolyCOOH),通过固体核磁、ζ-电势及元素分析对固定相进行了表征。选取核苷和核酸碱为模型化合物,通过考察流动相组成,离子强度和pH等因素对溶质保留的影响,探讨了固定相的分离性能和保留机理,结果表明,该固定相的保留机理同时涉及亲水分配相互作用和多重主客体作用力。该固定相还对糖类、敌草快与百草枯等化合物具有良好的分离性能。上述研究结果表明该固定相在极性化合物的分离上具有良好的应用前景。a weak cationic exchange/hydrophilic interaction mixed-mode stationary phase(Sil-PolyCOOH) for high performance liquid chromatography was prepared by bonding polyethylene maleic anhydride to the surface of amino silica gel via a nucleophilic substitution reaction, followed by hydrolysis of the residual maleic anhydride. The new stationary phase material was characterized by solid-state13C nuclear magnetic resonance analysis and ζ-potential measurements. The chromatographic performance and retention mechanism of this stationary phase were evaluated using nucleosides and nucleic bases as probes. The effects of mobile phase composition, ion strength, and pH on the retention factor were investigated. The results show that the retention mechanism of the stationary phase involves both hydrophilic distribution interaction and multiple forces between the host and guests. The stationary phase also showed good separation performance for saccharides, diquat, and paraquat. These results show that the stationary phase has a good application prospect in the separation of polar compounds.

关 键 词：亲水相互作用色谱 高效液相色谱 弱阳离子交换 聚乙烯马来酸酐 固定相 

分 类 号：O658[理学—分析化学]