替格瑞洛对脓毒症大鼠急性肾损伤时血浆P选择素浓度的影响  

Effect of ticagrelor on plasma P-selectin concentration during acute kidney injury in septic rats

作　　者：于澄 谢娜 高翠敏 马玉清[1] Yu Cheng;Xie Na;Gao Cuimin;Ma Yuqing(Department of Anesthesiology,First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一医院麻醉科,730000

出　　处：《中华麻醉学杂志》2019年第10期1240-1242,共3页Chinese Journal of Anesthesiology

基　　金：兰州大学第一医院院内基金项目(ldyyyn2018-49)。

摘　　要：目的评价替格瑞洛对脓毒症大鼠急性肾损伤时血浆P选择素(CD62P)浓度的影响。方法SPF级健康雄性SD大鼠30只,6~8周龄,体重180~220 g,采用随机数字表法分为3组(n=10):假手术组(S组)、脓毒症组(Sep组)和替格瑞洛组(T组)。采用盲肠结扎穿孔法制备大鼠脓毒症模型,S组麻醉开腹翻动肠管后关腹。T组于制备模型后12 h时给予替格瑞洛8.6 mg/kg灌胃,S组和Sep组在上述时点给予等量蒸馏水灌胃。于制备模型后24 h时取心脏血样测定Scr和血浆CD62P浓度。随后处死大鼠取双侧肾组织,HE染色观察肾脏病理学结果,Annexin/V-FITC双染细胞后流式检测并通过荧光显微镜观察肾细胞凋亡情况。结果与S组比较,Sep组和T组Scr和血浆CD62P浓度、肾脏Paller评分及肾细胞凋亡率升高(P<0.05);与Sep组比较,T组Scr和血浆CD62P浓度、肾脏Paller评分及肾细胞凋亡率降低(P<0.05)。结论替格瑞洛减轻脓毒症大鼠急性肾损伤的机制与降低血浆CD62P浓度,抑制细胞凋亡有关。Objective To evaluate the effect of ticagrelor on plasma P-selectin(CD62P)concentration during acute kidney injury in septic rats.Methods Thirty SPF healthy male Sprague-Dawley rats,aged 6-8 weeks,weighing 180-220 g,were divided into 3 groups(n=10 each)using a random number table method:sham operation group(group S),sepsis group(group Sep)and ticagrelor group(group T).Sepsis was induced by cecal ligation and puncture in anesthetized rats.The abdomen was opened after anesthesia induction and then closed after turning the intestines in group S.Ticagrelor 8.6 mg/kg was given through a gastric tube into stomach at 12 h after establishing the model in group T,while the equal volume of distilled water was given instead in S and Sep groups.Blood samples were collected from hearts at 24 h after establishing the model for determination of the concentrations of Scr and plasma CD62P.The rats were then sacrificed,and bilateral renal tissues were taken for examination of pathological changes and for determination of cell apoptosis by fluorescence microscopy after Annexin/V-FITC double staining was performed.Results Compared with group S,the concentrations of Scr and plasma CD62P,renal Paller score and apoptosis rate of renal cells were significantly increased in Sep and T groups(P<0.05).Compared with group Sep,the concentrations of Scr and plasma CD62P,renal Paller score and apoptosis rate of renal cells were significantly decreased in group T(P<0.05).Conclusion The mechanism by which ticagrelor reduces acute kidney injury is related to decreasing plasma CD62P concentrations and inhibiting cell apoptosis in septic rats.

关 键 词：血小板聚集抑制剂 脓毒症 急性肾损伤 P选择素 

分 类 号：R459.7[医药卫生—急诊医学]