蛴螬对激光损伤兔血-视网膜屏障后视网膜TekC、NGF表达的影响  

Effect of Lanthanum on the Expression of TrkC and NGF in the Retina of Rabbits with Laser-induced Retinal Barrier

作　　者：蒋鹏飞 杨霞 彭俊[2,4] 彭清华[1,2,4] Jiang Pengfei;Yang Xia;Peng Jun;Peng Qinghua(Hunan University of Traditional Chinese Medicine,Changsha 410208,China;First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China;Hubei Aerospace Hospital,Wuhan 432000,China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha 410208,China)

机构地区：[1]湖南中医药大学中医学院,长沙410208 [2]湖南中医药大学第一附属医院,长沙410007 [3]湖北航天医院,武汉432000 [4]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,长沙410208

出　　处：《世界科学技术-中医药现代化》2019年第11期2508-2515,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会青年科学基金项目(81804150):补虚活血中药对虚中夹瘀证RP模型大鼠感光细胞凋亡相关基因表达的干预作用及机制,负责人:彭俊;中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018):中医药防治五官科疾病湖南省重点实验,负责人:彭清华;长沙市科学技术局重大专项(K1501014-31,kc1704005):中医药防治眼耳鼻咽喉疾病湖南省重点实验,负责人:彭清华。

摘　　要：目的探讨蛴螬促进激光损伤兔血-视网膜屏障后的修复作用及机理。方法 30只实验性兔随机分为正常组、模型组、蛴螬组。采用激光损伤血-视网膜屏障动物模型,在屏障损伤后1周及2周两个时相,观察蛴螬对兔血-视网膜屏障损伤修复情况、血-视网膜屏障组织形态结构及神经营养因子受体TrkC、NGF表达的影响。结果蛴螬能促进损伤的血-视网膜屏障修复,减轻激光导致的视网膜组织及细胞形态学损伤,促进TrkC、NGF的表达,抑制视网膜细胞增殖与变性,从而改善视网膜功能。结论蛴螬可能通过改善视网膜组织能量代谢,清除氧自由基,促进神经营养因子受体的表达,抑制视细胞增殖与变性,起到保护血-视网膜屏障组织结构的作用。Objective To explore the role of sputum in promoting the repair of laser-damaged rabbit blood-retinal barrier and its mechanism. Methods Thirty rabbits were randomly divided into normal control group, model control group and sputum group. Laser-damaged blood-retinal barrier animal model was used to observe the repair of bloodretinal barrier damage, blood-retinal barrier tissue morphology and neurotrophin receptor TrkC in rabbits at 1 week and2 weeks after barrier injury. NGF expression and influence. Results It can promote the repair of blood-retinal barrier damage, reduce the retinal tissue and cell morphology damage caused by laser, promote the expression of TrkC and NGF,inhibit the proliferation and degeneration of retinal cells, and improve the retinal function. Conclusions It may protect the blood-retinal barrier tissue structure by improving energy metabolism of retinal tissue, scavenging oxygen free radicals, promoting the expression of neurotrophin receptors, inhibiting the proliferation of visual cells.

关 键 词：蛴螬 激光 血-视网膜屏障(BRB) 神经营养因子受体(TrkC、NGF) 

分 类 号：R285.1[医药卫生—中药学]