益气活血中药配伍对间歇性低氧复合胰岛素抵抗细胞模型SREBP-1c/FAS信号通路的干预效应  

Effects and the Mechanism of Chinese Medicine Supplementing Qi and Activating Blood Circulation on Intermittent Hypoxia Composite Insulin Resistance Cell Model

作　　者：马林沁 张京春[1,2] 刘玥[1,2] 乔羽 毛婷 雷舒雁 郑乔仙 孙欣丽 Ma Linqin;Zhang Jingchun;Liu Yue;Qiao Yu;Mao Ting;Lei Shuyan;Zheng Qiaoxian;Sun Xinli(Cardiovascular Center,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;Institute of Cardiovascular Diseases,China Academy of Chinese Medical Sciences,Beijing 100091,China;Cardiopulmonary Division,Beijing Mentougou District TCM Hospital,Beijing 102300,China;Graduate School,Beijing University of Chinese Medicine,Beijing 100020,China)

机构地区：[1]中国中医科学院西苑医院心血管病中心,北京100091 [2]中国中医科学院心血管病研究所,北京100091 [3]北京市门头沟区中医院心肺科,北京102300 [4]北京中医药大学研究生院,北京100020

出　　处：《世界科学技术-中医药现代化》2019年第11期2516-2525,共10页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会面上项目(81573871):益气活血中药对慢性间歇性低氧复合胰岛素抵抗介导的动脉粥样硬化过程中SREBP-1c/FAS通路的影响,负责人:张京春;国家自然科学基金委员会面上项目(81373825):慢性间歇性低氧对高血压血管内皮p38MAPK/NF-kB信号通路的影响及清眩颗粒的干预机制研究,负责人:张京春;北京市科学技术委员会科技新星人才培养专项计划(Z171100001117027):中西医结合临床医学,负责人:刘玥。

摘　　要：目的研究益气活血中药单体人参皂苷Re联合川芎嗪(TMP)对间歇性低氧(IH)复合胰岛素抵抗(IR)的3T3-L1脂肪细胞模型的干预效应,并观察SREBP-1c/FAS信号通路在其中的作用。方法实验采用IH复合IR的3T3-L1脂肪细胞模型,分别以人参皂苷Re及TMP作为干预药物,首先进行中药最优配伍浓度体外筛选,然后选择最优配伍浓度作为干预剂量,观察其对模型细胞SREBP-1c/FAS信号通路的干预效应。结果人参皂苷Re 1μmol·L-1联合TMP 10μmol·L-1时,对IH条件下SREBP-1c、FAS mRNA表达水平抑制最明显。该单体配伍及Betulin能抑制IH引起的SREBP-1蛋白表达水平上调,升高IH条件下IRS-1、SOD mRNA表达水平,降低IH条件下HIF-1αmRNA表达水平。结论人参皂苷Re与TMP体外联合作用能抑制IH对IR-3T3-L1细胞SREBP-1c/FAS通路表达水平的上调作用,且有一定的改善IR、氧化应激的效应。Objective To explore the interventional effects of ginsenoside Re combined with Ligustrazine(TMP) on intermittent hypoxia(IH) composite insulin resistance(IR) cell model of 3 T3-L1 adipocytes in vitro, and to observe the effect of SREBP-1 c/FAS signal pathway. Methods IH composite IR cell model of 3 T3-L1 adipocytes was built. The effective components of Chinese medicine of supplementing Qi and activating blood circulation including ginsenoside Re combined with ligustrazine(TMP) were used as intervention drugs. The optimal compatibility concentration of them was screened in vitro, which was selected as the intervention dose to observe the effect on SREBP-1 c/FAS signaling pathway in IH composite IR 3 T3-L1 adipocytes model. Results The combination the concentration of Re 1 μmol·L-1 with TMP10 μmol·L-1 inhibited the mRNA expression of SREBP-1 c and FAS after IH treatment. The drug inhibited IHinduced upregulation of protein expression of SREBP-1 and FAS, increased the mRNA expression level of IRS-1 and SOD and reduced the mRNA expression level of HIF-1α after IH treatment. Conclusion The study demonstrates the combination of ginsenoside Re and TMP has the effect of inhibiting expression of IH-induced SREBP-1 c/FAS pathway in IR-3 T3-L1 adipocytes and may have the effect of improving IR and oxidative stress.

关 键 词：间歇性低氧 胰岛素抵抗 3T3-L1脂肪细胞 益气活血 

分 类 号：K2-031[历史地理—历史学]