先玉335、郑单958和京科968抗虫能力的比较研究  

Comparation of Herbivore Resistance among the Maize Lines Xianyu335, Zhengdan958, and Jingke968

作　　者：刘树楠 申国境 李京 张翠萍 Saif-UL-Mallook 吴建强 王蕾 宋伟[3] 王燚 赵久然[3] 唐国文[1] 齐金峰 LIU Shu-nan;SHEN Guo-jing;LI Jing;ZHANG Cui-ping;Saif-UL-Mallook;WU Jian-qiang;WANG Lei;SONG Wei;WANG Yi;ZHAO Jiu-ran;TANG Guo-wen;QI Jin-feng(College of Plant Protection,Yunnan Agricultural University,Kunming 650201;Department of Economic Plants and Biotechnology,Yunnan Key Laboratory for Wild Plant Resources,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201;Maize Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]中国科学院昆明植物研究所/资源植物与生物技术重点实验室/云南省野生资源植物研发重点实验室,昆明650201 [3]北京市农林科学院玉米研究中心,北京100097

出　　处：《玉米科学》2019年第6期52-57,共6页Journal of Maize Sciences

基　　金：国家自然科学基金(31772179);云南省应用基础研究计划(2018FB025)

摘　　要：以先玉335、郑单958、京科968为材料,以玉米螟(Ostrinia furnacalis)、黏虫(Mythimna separata)和禾谷缢管蚜(Rhopalosiphum padi)为供试昆虫,探究玉米品系大喇叭口期对玉米螟和黏虫及抽雄期对禾谷缢管蚜抗性差异,测定其主要抗虫化合物丁布类物质的含量。结果发现,京科968对玉米螟及禾谷缢管蚜抗性均高于另外两个品系;对黏虫的抗性,郑单958和京科968高于先玉335。大喇叭口期京科968中丁布类物质含量最高,抽雄期郑单958和京科968中主要丁布成分含量均高于先玉335,上述组织中丁布合成相关基因在郑单958及京科968中的表达量也均高于先玉335。本研究揭示杂交玉米抗虫性与抗性相关化合物含量及其相关基因表达间存在正向相关关系,为抗虫玉米分子育种提供理论参考。In order to investigate the herbivore resistance of commercial hybrid maize lines in China, we determined late-whorl stage and tasseling-stage commercial maize lines Xianyu335, Zhengdan958 and Jingke968 for their resistance to Ostrinia furnacalis, Mythimna separata, and Rhopalosiphum padi, and the contents of the major defense-related secondary metabolites-benzoxazinoids(Bxs) were quantified. The results showed that Jingke968 was more resistant to O. furnacalis and R. padi than the other two maize lines, while Zhengdan958 and Jingke968 showed higher levels of resistance to M. separata than did Xianyu335. Accordingly, the leaves of Jingke968 had higher Bxs levels at the late-whorl stage than did the other two maize lines. The major Bxs levels in leaves and stems in Zhengdan958 and Jingke968 were higher than in Xianyu335 at the tasseling stage. The transcript levels of Bx biosynthesis-related genes in Zhengdan958 and Jingke968 were higher than those in Xianyu335. This study reveals the positive correlation between maize herbivore resistance and defensive secondary metabolites as well as the transcript levels of corresponding biosynthesis-related genes which provide knowledge on herbivore-resistance maize breeding.

关 键 词：玉米 玉米螟 黏虫 禾谷缢管蚜 抗虫能力 丁布 

分 类 号：S513.037[农业科学—作物学]