环烷基磺酰胺类化合物对拟轮枝镰孢菌抑制作用及伏马菌素生物合成基因表达的影响  

Inhibition Effect of Cycloakyl Sulfonamide on Fusarium verticillioides and Gene Expression of Fumonisins Biosynthesis

作　　者：许佳宁[1] 李兴海[1] 马昌广 孙佳莹[1] 薛春生[1] 肖淑芹[1] XU Jia-ning;LI Xing-hai;MA Chang-guang;SUN Jia-ying;XUE Chun-sheng;XIAO Shu-qin(College of Plant Protection,Shenyang Agricultural University,Shenyang 110161;Institute of Crop Sciences,Dalian Academy of Agricultural Sciences,Dalian 116036,China)

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]大连市农业科学院作物科学研究所,辽宁大连116036

出　　处：《玉米科学》2019年第6期160-166,共7页Journal of Maize Sciences

基　　金：辽宁省高等学校基本科研项目(2017018)

摘　　要：采用菌丝生长速率法,以噁霉灵原药为对照药剂,从62种环烷基磺酰胺类化合物中筛选出436、MSQ8和WML-1等3种对拟轮枝镰孢菌菌丝生长有明显抑制作用的化合物,其EC50分别为11.42μg/mL、20.85μg/mL和33.76μg/mL,高于或接近于对照药剂噁霉灵的EC50(20.77μg/mL)。对拟轮枝镰孢菌分生孢子萌发抑制进行试验,结果表明,化合物WML-1、MSQ8和436的EC50分别为165.25μg/mL、185.12μg/mL和274.46μg/mL,均低于对照药剂噁霉灵的EC50(65.81μg/mL)。qRT-PCR结果表明,3种药剂处理后对伏马菌素的生物合成基因表达无明显影响。Mycelium growth rate of Fusarium verticillioides was tested by hymexazol and 62 novel cycloalkyl sulfonyl compounds.Among them,compounds 436,MSQ8 and WML-1 were three kinds of the most effective compounds that could inhibit the mycelium growth of F.verticillioodes,with the EC50 values of 11.42μg/mL,20.77μg/mL and 33.76μg/mL,respectively.Inhibit effect was higher than or close that of control fungicide.They were not demonstrated inhibitory effects on conidia germination,with the EC50 values of 165.25μg/mL,185.12μg/mL and 274.46μg/mL,inhibit effect was lower than that of control fungicide.qRT-PCR results showed that 3 compounds did not inhibit fumonisins biosynthesis.

关 键 词：玉米 环烷基磺酰胺类化合物 拟轮枝镰孢菌 伏马菌素 实时定量PCR 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]