玉米黑粉菌多糖的提取及其对小鼠急性酒精肝损伤保护作用的研究  

Study on the Extraction of Polysaccharide from Ustilago maydis and Its Protective Effect on Acute Alcoholic Liver Injury in Mice

作　　者：张永红 张杰 张桥 荣林 孙永全 吕文旭 柏瑞彤 Zhang Yonghong;Zhang Jie;Zhang Qiao;Rong Lin;Sun Yongquan;Lv Wenxu;Bai Ruitong(Qilu Medical University,Zibo 255300,China)

机构地区：[1]齐鲁医药学院

出　　处：《成都医学院学报》2020年第1期50-53,共4页Journal of Chengdu Medical College

基　　金：齐鲁医药学院基金项目(No:X17ZK11)

摘　　要：目的研究玉米黑粉菌多糖对小鼠急性酒精肝损伤的保护作用。方法60只昆明小鼠,随机分为空白组、模型组、阳性组[联苯双酯(150mg/kg)]和玉米黑粉菌高糖组(400mg/kg)、中糖组(200mg/kg)、低糖组(100mg/kg),每组各10只。除空白组、模型组予以生理盐水外,其他各组按20mL/kg灌胃给药,30d后,除空白组外,其余5组按12mL/kg给予50%酒精,建立急性酒精肝模型,禁食不禁水16h,眼球取血,收集血清。以血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(AKP)的含量及病理切片来分析玉米黑粉菌多糖对急性酒精肝损伤小鼠的作用效果。结果与模型组比较,玉米黑粉菌高糖组明显降低小鼠血清中ALT、AST、AKP的含量,能明显减轻肝脏变性和坏死等病理改变;但是玉米黑粉菌中糖组、低糖组小鼠血清中ALT、AST、AKP的含量无明显改变,肝脏变性等也未明显改善。结论玉米黑粉菌多糖对小鼠急性酒精肝损伤保护作用明显。Objective To study the protective effect of Ustilago maydis polysaccharide on acute alcoholic liver injury in mice.Methods A total of 60 mice were randomly divided into the blank group,model group,positive group with 150 mg/kg Bifendatatum,and high-,medium-and low-dose groups with 400 mg/kg,200 mg/kg and100 mg/kg Ustilago maydis polysaccharide respectively.The blank group and model group were given normal saline,while the other groups were administered intragastrically by 20 ml/kg.All the groups except the blank group were given 50%alcohol by 20 ml/kg to establish the acute alcoholic liver model.After 16-hour fasting without water deprivation,the blood was taken from eyeballs to collect serum.The effect of Ustilago maydis polysaccharide on acute alcoholic liver injury in mice was evaluated by analyzing the content of ALT,AST and AKP in serum and the pathological sections.Results Compared with the model group,the content of ALT,AST and AKP in serum was significantly decreased and the liver degeneration and necrosis were significantly alleviated in the high-dose polysaccharide group,while there were no significant changes in the content of ALT,AST and AKP in serum and no significant improvement in liver degeneration in the medium-and low-dose polysaccharide groups.Conclusion The polysaccharide extracted fromUstilago maydis has obvious protective effect on acute alcoholic liver injury in mice.

关 键 词：玉米黑粉菌 多糖 急性酒精肝损伤 

分 类 号：R961.1[医药卫生—药理学]