静电轨道离子阱离子切向引入的新方式和模拟  

New Method of Tangential Ion Injection into Electrostatic Orbitrap with Simulation

作　　者：刘颖超 刘嘉琳 申华莉 杨芃原[1,2] LIU Ying-chao;LIU Jia-lin;SHEN Hua-li;YANG Peng-yuan(Institutes of Biomedical Sciences,Fudan University,Shanghai,200032,China;Engineering Center of Ministry of Education for Innovation Scientific Instruments,Department of Chemistry,Fudan University,Shanghai,200438,China)

机构地区：[1]复旦大学生物医学研究院,上海200032 [2]复旦大学化学系,创新科学仪器教育部工程中心,上海200438

出　　处：《质谱学报》2020年第2期160-167,I0006,共9页Journal of Chinese Mass Spectrometry Society

基　　金：国家重大科研仪器研制项目(81827901);国家卫健委专项(YS2017YFSF090099)资助。

摘　　要：静电轨道离子阱质谱仪的关键垄断技术是将离子引入静电轨道离子阱的C形离子阱。本工作提出一种新的离子引入方式,即设计了一种O形离子阱,用于将更多的离子以较少的损失引入到静电离子阱中进行分析。O形离子阱嵌套在静电轨道离子阱外轨道上,可以直接使离子从O形圆轨道下滑降轨内切至椭圆轨道,再沿椭圆轨道下滑降轨外切,最终射入静电轨道离子阱中的圆轨道。新的离子引入方式避免了C形离子阱远距离传输离子,离子流可连续进入O形圆轨,在脉冲电压作用下进入静电轨道离子阱;随着离子的引入面增大,离子的引入量有所增加。另外,还推导了离子运动轨迹方程及降轨脉冲的能量方程,对离子引入方式进行模拟,结果表明,多离子多位置同时引入对离子轨迹无明显影响,而离子是否切向引入则至关重要,偏离切向引入会大大降低离子寿命。The key monopoly technique of electrostatic orbitrap mass spectrometer is to inject ions from the C-trap into Orbitrap. In this work, a new method of ion injection with an O-shaped ion trap was presented. The O-trap was located on the outer orbit of electrostatic Orbitrap. Firstly, ions were introduced tangentially into O-trap for circular motion, and then by controlling the voltage between the O-trap and Orbitrap electrodes, ions could be directly transferred from the circular O-trap down to an elliptical orbit with a lower potential. Ions travelled downward along the elliptical orbit, and eventually down-injected into the static circle Orbitrap. The method allowed the ion flue ionized by ion source to enter continuously into the circular O-trap and to repulse into static Ob-trap, avoiding the long-distance transmission of ions from the C-trap. The ions inject volume therefore increased with more ions being injected. The equation of ion motion trajectory had been derived and the energy equation of the applied pulse was established to alter ion orbital. According to the equation, for the elliptical motion after the descent, its long axis(2a) was determined by the geometric dimensions of the electrostatic Orbitrap and the outer O-trap, and its length of the short axis(2b with a^2=b^2+c^2, c is the focal length of ellipse) was related to the strength and duration of the pulse voltage. The variable-orbit pulse voltage ΔU was determined only by the differential voltage applied to the respective internal and external electrodes of Orbitrap and O-trap. Meanwhile, the method of ion introduction was also simulated in this study. The simulation results showed that the angle of incidence was equal when multiple ions were tangent inward at each point of the outer O-trap due to the symmetry of the electrostatic ion trap device. Thus, no obvious effect was found for multi-ion and multi-position injection on ion trajectory, but the tangential ion injection was very critical, and any deviation from tangent injection would grea

关 键 词：静电轨道离子阱 O形离子阱 切向离子引入 离子轨迹模拟 

分 类 号：O657.63[理学—分析化学]