一测多评法同时测定解郁胶囊中9种成分  

Simultaneous determination of nine constituents in Jieyu Capsules by QAMS

作　　者：靳晓峰 魏谭军[3] 马伟丽 余晓晖[2] JIN Xiao-feng;WEI Tan-jun;MA Wei-li;YU Xiao-hui(The Hospital Affiliated to Gansu University of Chinese Medicine,Lanzhou 730000,China;Gansu University of Chinese Medicine,Lanzhou 730000,China;Dazhou Municipal Hospital of Integrated Traditional Chinese Medicine&Western Medicine,Dazhou 635000,China)

机构地区：[1]甘肃中医药大学附属医院,甘肃兰州730000 [2]甘肃中医药大学,甘肃兰州730000 [3]达州市中西医结合医院,四川达州635000

出　　处：《中成药》2020年第3期574-579,共6页Chinese Traditional Patent Medicine

摘　　要：目的建立一测多评法同时测定解郁胶囊(丹参、柴胡、石菖蒲等)中氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷、柴胡皂苷a、柴胡皂苷d、丹参素、丹酚酸B、丹参酮ⅡA的含有量。方法该药物70%甲醇提取液的分析采用Agilent Extend-C18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量0.9 mL/min;柱温30℃;检测波长210、230、280 nm。以芍药苷为内标,计算其他8种成分的相对校正因子,测定其含有量。结果 9种成分在各自范围内线性关系良好(r≥0.999 1),平均加样回收率96.97%~100.18%,RSD 0.60%~1.51%,一测多评所得结果与外标法接近。结论该方法稳定可靠,可用于解郁胶囊的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS) method for the simultaneous content determination of oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, saikosaponin a, saikosaponin d, danshensu, salvianolic acid B and tanshinone ⅡA in Jieyu Capsules(Salviae miltiorrhizae Radix et Rhizoma, Bupleuri Radix, Acori tatarinowii Rhizoma, etc.).METHODS The analysis of 70% methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent Extend-C18 column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 0.9 mL/minin a gradient elution manner, and the detection wavelengths were set at 210, 230, 280 nm. Paeoniflorin was used as an internal standard to calculate the relative correction factors of the other eight constituents, after which the content determination was made.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.999 1), whose average recoveries were 96.97%-100.18% with the RSDs of 0.60%-1.51%. The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This stable and reliable method can be used for the quality control of Jieyu Capsules.

关 键 词：解郁胶囊 化学成分 一测多评 

分 类 号：R927[医药卫生—药学]