针刺预处理对荨麻疹大鼠血清IgE及皮肤组织中磷酸化酪氨酸蛋白激酶表达的影响  

Effect of pre-acupuncture intervention on serum IgE and cutaneous phosphorylated tyrosine-protein kinase expression in rats with urticaria

作　　者：王月铭 马铁明 WANG Yue-ming;MA Tie-ming(College of Acupuncture and Tuina,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学针灸推拿学院

出　　处：《针刺研究》2020年第2期111-116,共6页Acupuncture Research

基　　金：辽宁省针灸养生康复重点实验室;辽宁省教育厅创新团队项目(No.LT2015016)

摘　　要：目的:观察针刺预处理对荨麻疹大鼠血清IgE水平及背部待测区域皮肤组织肥大细胞脱颗粒,组胺和5-羟色胺含量,磷酸化酪氨酸蛋白激酶Lyn、Syk(p-Lyn、p-Syk)表达的影响,探讨针刺预防和治疗荨麻疹的作用机制。方法:SD大鼠随机分为正常对照组、模型对照组、阳性药物组、针刺组,每组10只。采用抗卵蛋白血清致敏建立大鼠荨麻疹模型。造模前14 d,阳性药物组大鼠予以氯雷他定灌胃(0.1 mg/100 g),针刺组给予针刺双侧"曲池""血海",留针30 min,每日1次,两组均连续干预14 d。用HE染色法观察大鼠皮肤组织形态变化;ELISA法检测血清总IgE水平;甲苯胺蓝染色法观察皮肤组织肥大细胞脱颗粒情况;免疫组织化学法检测皮肤组织组胺和5-羟色胺表达及p-Lyn和p-Syk的表达。结果:与正常对照组比较,模型对照组大鼠的表皮明显增厚,真皮肿胀,小血管炎性浸润严重,肥大细胞肿胀变形,边缘轮廓模糊,有颗粒脱出,血清IgE水平明显升高(P<0.05),皮肤组织组胺、5-羟色胺表达及p-Lyn、p-Syk表达明显增加(P<0.05)。与模型对照组比较,针刺组和阳性药物组大鼠的表皮略有增厚,真皮轻度水肿,偶有炎性细胞,肥大细胞脱颗粒现象减少,血清IgE水平明显降低(P<0.05),皮肤组织组胺、5-羟色胺及p-Lyn和p-Syk表达明显降低(P<0.05)。针刺组和阳性药物组各指标差异无统计学意义(P>0.05)。结论:针刺"曲池""血海"穴可以防治荨麻疹,抑制Ⅰ型超敏反应及肥大细胞脱颗粒,其机制可能与针刺对皮肤组织p-Lyn、p-Syk表达的调控有关。Objective To observe the effect of acupuncture on serum IgE level, the degranulation of mast cells, the release of histamine and serotonin, and the expressions of phosphorylated tyrosine-protein kinase Lyn and Syk(p-Lyn, p-Syk) in skin tissue in rats with urticaria, as well as analyze the mechanism of acupuncture in the prevention and the treatment of urticaria. Methods SD male rats were randomly divided into normal control, model control, medication and acupuncture groups(n=10 in each group). The anti-ovalbumin serum was used to establish urticaria model. Rats of the medication group received gastric lavage of Loratadine(0.1 mg/100 g). In the acupuncture group, bilateral "Xuehai"(SP10) and "Quchi"(LI11) were punctured perpendicularly, about 2 to 4 mm in depth, and the needles were retained for 30 min. The treatment was given consecutively for 14 days in the two treatment groups. H.E. staining was adopted to observe the morphological changes of skin tissue, ELISA to determine the total IgE level in serum, the toluidine blue staining to observe the degranulation of mast cells in local skin tissue and the immunohistochemistry to determine the expressions of histamine and serotonin as well as the the expressions of p-Lyn and p-Syk. Results Compared with the normal control group, the epidermis of the model control group was significantly thickened, the dermis was swollen, the inflammatory infiltration of small vessels was serious and the mast cells were swollen and deformed, with blurred edge and exfoliated granules. Additionally, in the model control group, the serum IgE level was significantly higher(P<0.05), the expressions of histamine and serotonin, as well as p-Lyn and p-Syk proteins in local skin tissue were significantly increased(P<0.05). Compared with the model control group, in the acupuncture group and the medication group, the epidermis was slightly thickened, the dermis got slightly edema, the inflammatory cells were presented occasionally and the degranulation of mast cells were reduced. Besides, the s

关 键 词：针刺 荨麻疹 IGE 肥大细胞 组胺 5-羟色胺 磷酸化酪氨酸蛋白激酶Lyn 磷酸化酪氨酸蛋白激酶Syk 

分 类 号：R245.31+1[医药卫生—针灸推拿学]