艾灸对哮喘大鼠“肺俞”穴局部皮肤中组胺和神经肽的影响  

Effect of moxibustion on respiratory function and cutaneous histamine and neuropeptide contents of “Feishu” (BL13) in asthmatic rats

作　　者：张国山[1,2] 邱冉冉 潘江[3] 张建[2] 张驰 王诚喜[2] 阳仁达 ZHANG Guo-shan;QIU Ran-ran;PAN Jiang;ZHANG Jian;ZHANG Chi;WANG Cheng-xi;YANG Ren-da(College of Acupuneture moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha 410208,China;Department of Pulmonary Disease,Hengyang Hospital of Chinese Medicine,Hengyang 421001,Hunan Province;The First Hospital,Hunan University of Chinese Medicine,Changsha 410007;Graduate School,Hunan University of Chinese Medicine,Changsha 410208)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208 [2]衡阳市中医医院肺病科,湖南衡阳421001 [3]湖南中医药大学第一附属医院,长沙410007 [4]湖南中医药大学研究生院,长沙410208

出　　处：《针刺研究》2020年第2期117-121,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81603705、81774438);中国博士后科学基金项目(No.2107M612567);湖南省自然科学基金青年项目(No.2017JJ3245、2017JJ3243);湖南省教育厅重点项目(No.18A223)

摘　　要：目的:观察艾灸对哮喘大鼠呼吸功能、"肺俞"穴局部皮肤中组胺和神经肽含量的影响,探讨艾灸治疗哮喘的作用机制。方法:SD大鼠随机分为正常组、模型组和艾灸组,每组12只。卵清蛋白致敏并激发制备大鼠哮喘模型。激发的同时每天温和灸艾灸组大鼠"肺俞"穴,每次15 min,每天1次,连续干预14 d。用小动物肺功能测定仪测定各组大鼠的吸气阻力、呼气阻力和肺通气顺应性,酶联免疫吸附法检测"肺俞"穴局部皮肤中组胺和神经肽的含量。结果:与正常组相比,模型组大鼠的吸气阻力和呼气阻力均显著增加(P<0.01),肺通气顺应性显著降低(P<0.01);"肺俞"穴局部皮肤中血管活性肠肽(VIP)的含量显著降低(P<0.01),组胺、P物质(SP)、降钙素基因相关肽(CGRP)的含量均显著升高(P<0.01)。与模型组相比,艾灸组大鼠的吸气阻力和呼气阻力均显著降低(P<0.01),肺通气顺应性显著增高(P<0.01);"肺俞"穴局部皮肤中VIP的含量显著升高(P<0.05),组胺、SP、CGRP的含量均显著降低(P<0.01)。结论:艾灸"肺俞"穴具有调节肺功能的作用,其机制可能与调节"肺俞"穴局部皮肤中组胺和VIP、SP、CGRP的含量有关。Objective To observe the effect of moxibustion on respiratory function and contents of histamine and neuropeptides in skin tissue of "Feishu"(BL13) in asthmatic rats, so as to explore its mechanisms underlying improvement of asthma.Methods Thirty-six SD rats were randomly divided into normal control, model and moxibustion groups, with 12 rats in each group. The asthma model was established by subcutaneous injection(at the back and groin, 0.5 mL) and i.p. injection(1 mL)of mixture solution of ovalbumin(OVA), Aluminium Hydroxide gel and 0.9% sodium chloride solution, and repeated nasal drip of 1% OVA. Moxibustion was applied to bilateral BL13 for 15 min, once daily for 14 days. The inspiratory and expiratory resistance, and pulmonary ventilation compliance were detected by using a small animal pulmonary function tester under anesthesia. The contents of histamine, vasoactive intestinal peptide(VIP), substance P(SP) and calcitonin gene-related peptide(CGRP) in the local skin tissue of the left BL13 were assayed by using enzyme-linked immunosorbent assay(ELISA). Results After modeling, the inspiratory and expiratory resistance were significantly increased(P<0.01), and the lung ventilation compliance was significantly decreased in the model group relevant to the normal control group(P<0.01). Outcomes of ELISA showed that the content of VIP was significantly lower(P<0.01), and those of histamine, SP and CGRP in the skin of left BL13 were significantly increased in the model group relevant to the normal control group(P<0.01). Following the intervention, the inspiratory and expiratory resis-tance, cutaneous histamine, SP and CGRP contents were significantly decreased(P<0.01), while the lung ventilation compliance and cutaneous VIP level were significantly increased in the moxibustion group in contrast to the model group(P<0.01, P<0.05). Conclusion Moxibustion of BL13 can improve pulmonary function in asthma rats, which may be related to changes of levels of histamine and neuropeptides as VIP, SP and CGRP in the local skin

关 键 词：艾灸 哮喘 肺功能 皮肤 组胺 神经肽 

分 类 号：R245.81[医药卫生—针灸推拿学]