自身免疫病病人外周血NK细胞检测的意义  

Significance of NK cells in peripheral blood of the patients with autoimmune diseases

作　　者：陈琳[1] 吕程 孟天犁 姜国平[1] 李慧颖[1] 吕英春[1] 江涛 孔令环[1] CHEN Lin;LV Cheng;MENG Tian-li(Department of Rheumatology JiLin Province Hospital,Changchun 130021,China)

机构地区：[1]吉林省人民医院风湿免疫科,吉林长春130021

出　　处：《中国实验诊断学》2020年第2期266-269,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅项目(20150101145JC)。

摘　　要：目的探讨自身免疫病病人外周血NK(CD3-CD16+CD56+)细胞在自身免疫病发病机制中的作用。方法用流式细胞术检测29例自身免疫病病人和18例健康对照者外周血NK(CD3-CD16+CD56+)细胞数,同时检测NK细胞的胞膜活化性受体NKG2D(CD314)和抑制性受体NKG2A(CD159)以及穿孔素、颗粒酶-β的表达。结果自身免疫病病人外周血NK细胞数与健康对照组无明显差别。自身免疫病病人NK细胞膜活化性受体NKG2D和颗粒酶-β的表达在治疗前和治疗后均低于正常对照组(P<0.05);自身免疫病病人NK细胞膜膜抑制性受体NKG2A和穿孔素的表达在治疗前和治疗后与正常对照组相比,均无明显差别。结论外周血NK细胞的膜活化性受体NKG2D和颗粒酶-β表达减少可能与自身免疫病的发生、发展有关。Objective To explore the mechanism of NK(CD3-CD16+CD56+)cells in peripheral blood of the patients with autoimmune diseases.Methods The percentage of NK(CD3-CD16+CD56+)cells in peripheral blood of29 patients with autoimmune diseases and 18 healthy controls were detected by flow cytometry.And we also detected the expression levels of activated receptors NKG2 D(CD314),inhibitory receptors NKG2 A(CD159),perforin and granzymeβin NK cells.Results The expression of NK cells in peripheral blood of he patients with autoimmune diseases had no significant than that of control group.Compared with control group,the expression of NKG2 Din the patients with autoimmune diseases before and after treatment were significantly decreased(P <0.05);but NKG2 A and granzymeβhad no significant difference between the patients with autoimmune diseases and healthy controls.Conclusion The lower expression of NKG2 Aand granzymeβmay be involved in the pathogenesis of autoimmune diseases.

关 键 词：自身免疫病 NK细胞 NKG2D NKG2A 穿孔素 颗粒酶-β 

分 类 号：R446.6[医药卫生—诊断学]