无糖型银花感冒颗粒质量标准研究  

Study on Quality Standard of Sugar-free Yinhua Ganmao Granules(无糖型银花感冒颗粒)

作　　者：崔凯 贾朝 潘婷婷 周伟 孙金娟 程敏 CUI Kai;JIA Zhao;PAN Ting-ting;ZHOU Wei;SUN Jin-juan;CHENG Min(School of Biomedicine and Food Engineering,Shangluo University,Shangluo Shaanxi 726000,China;College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou Gansu 730000,China;Shaanxi Xiangju Pharmaceutical Group Co.,Ltd.,Shangluo Shaanxi 726000,China;College of Pharmacy,Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi 712000,China)

机构地区：[1]商洛学院生物医药与食品工程学院,陕西商洛726000 [2]西北师范大学化学化工学院,甘肃兰州730000 [3]陕西香菊药业集团有限公司,陕西商洛726000 [4]陕西中医药大学药学院,陕西咸阳712000

出　　处：《中医药导报》2020年第4期4-7,29,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省教育厅服务地方专项项目(18JC011)。

摘　　要：目的:建立无糖型银花感冒颗粒的质量标准。方法:采用薄层色谱(TLC)法定性鉴别复方制剂中桔梗、甘草和防风,采用高效液相色谱(HPLC)法测定复方制剂中的绿原酸和连翘苷的含量。结果:发现薄层色谱斑点分离度较好、清晰且阴性无干扰。绿原酸在0.076,8~1.150,2μg范围内有良好的线性关系(r=0.999,9),平均加样回收率为99.80%,RSD为1.45%。连翘苷在0.247,3~1.236,6μg范围内线性关系良好(r=0.999,9),平均加样回收率为98.20%,RSD为2.11%。结论:所建立的方法可靠准确,专属性强,重现性好,可作为无糖型银花感冒颗粒的质量控制方法。Objective: To establish the quality standard of sugar free Yinhua ganmao granules. Methods:Thin layer chromatography(TLC) was used to qualitative identify Jiegeng(platycodon), Gancao(licorice) and Fangfeng(saposhnikovia), and the content of chlorogenic acid and forsythin was determined by high performance liquid chromatography(HPLC). Results: TLC spots had a good resolution, clearer, and no interference. Chlorogenic acid has a good linear relationship in the range of 0.076,8-1.150,2 μg(r=0.999,9). The average recovery of chlorogenic acid is 99.8%, RSD is 1.45%. The linear relationship of forsythin in the range of 0.247,3-1.236,6 μg(r=0.999,9). The average recovery rate was 99.82%, RSD was 2.11%. Conclusion: The method is reliable, accurate, specific, and reproducible. It can be used as a quality control method for sugar-free Yinhua Ganmao granules.

关 键 词：无糖型银花感冒颗粒 桔梗 甘草 防风 绿原酸 连翘苷 

分 类 号：R284.1[医药卫生—中药学]