更舒方对去势围绝经期综合征模型大鼠的药效学研究  

Pharmacodynamic Study of Gengshufang(更舒方) on Castrated Perimenopausal Syndrome Model Rats

作　　者：孙亚 李萍[3] 高明周 李师谦[4] 乔明琦 SUN Ya;LI Ping;GAO Ming-zhou;LI Shi-qian;QIAO Ming-qi(School of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan Shandong 250355,China;The State Administration of Traditional Chinese Medicine Focuses on Cultivating the Research and Innovation Team of Emotional Diseases and Syndromes of Shandong University of Traditional Chinese Medicine,Jinan Shandong 250355,China;Affiliated Hospital of Qingdao University,Qingdao Shandong 266001,China;32125 Troops of the Chinese Peopled Liberation Army,Jinan Shandong 25000Z China)

机构地区：[1]山东中医药大学中医学院,山东济南250355 [2]国家中医管理局重点培育学科中医情志学学科山东中医药大学情志病证科研创新团队,山东济南250355 [3]青岛大学附属医院,山东青岛266001 [4]中国人民解放军32125部队,山东济南250002

出　　处：《中医药导报》2020年第4期30-34,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：重大新药创制(SQ2017ZX091064),项目名称:中药新药丹皮酚滴丸、柴芍皂苷软胶囊和贞芍醇苷胶囊的研发与其关键创新技术。

摘　　要：目的:观察更舒方对卵巢去势围绝经期综合征模型大鼠行为学特点、血清激素及子宫系数的影响,探讨更舒方针对围绝经期综合征的药效学特点。方法:采取卵巢去势法制备围绝经期综合征大鼠模型。将造模成功48只大鼠据体质量按随机区划法分为模型组、坤泰组、17-β雌二醇+孕酮(下称雌激素组)以及更舒方高、中、低剂量组,同时设假手术组作为对照组。雌激素组皮下注射给药,其余组灌胃给药,给药4周,于末次给药后24 h内进行旷场实验、高架十字迷宫实验及明暗箱实验,进行行为学评估;采用10%水合氯醛(30 mg/kg)腹腔麻醉后腹主动脉取血并切除子宫;采用ELISA法测定血清雌二醇(E2)、促黄体生成素(LH)、促卵泡激素(FSH)浓度;称双侧子宫湿质量,计算子宫系数。结果:旷场实验结果显示,更舒方高、低剂量组大鼠运动总路程长于模型组(P<0.01),雌激素组和更舒方中剂量组大鼠进入中央区次数增多(P<0.05),且雌激素组在中央区停留时间延长(P<0.01),更舒方低剂量组大鼠运动总路程长于坤泰组;高架十字迷宫实验结果显示,与对照组比较,模型组大鼠OE%低于对照组(P<0.05),雌激素组和更舒方高、中剂量组大鼠的OE%和OT%明显高于模型组(P<0.05),更舒方中剂量组大鼠OE%、OT%均高于坤泰组(P<0.01);更舒方高剂量组OT%高于坤泰组(P<0.01);明暗箱实验结果显示,模型组大鼠在明箱停留时间与对照组比较有减少的趋势,但差异无统计学意义(P>0.05),雌激素组和更舒方中剂量组大鼠在明箱停留时间明显长于模型组(P<0.05),舒更方低剂量组大鼠在明箱停留时间短于坤泰组(P<0.05)。血清激素水平及子宫系数结果显示,模型组大鼠血清FSH和LH含量明显高于对照组(P<0.05),子宫系数低于对照组(P<0.05);更舒方高、中剂量组大鼠血清E2含量高于模型组(P<0.05),FSH和LH含量低于模型组(P<0.05),子宫系数高于模型组(P<0.05),Objective: To observe the effects of Gengshufang on behavioral characteristics, serum hormones and uterine coefficient of ovarian castration-perimenopausal syndrome model rats, and to explore the pharmacodynamic characteristics of Gengshufang against perimenopausal syndrome. Methods: The perimenopausal rats models were established by ovarian castration. A total of 48 rats successfully modeled were divided into model group, Kuntai group, 17-βestradiol + progesterone(estrogen group) and Gengshufang high, medium and low-dose groups according to their body weight according to the random division method. The sham-operation group was set as the control group. The estrogen group was administered by subcutaneous injection, and the other groups were administered by intragastric administration for 4 weeks. The open field experiment, elevated cross labyrinth experiment, and light and dark box experiments were performed within 24 hours after the last administration.Blood was taken from the abdominal aorta after peritoneal anesthesia with 10% chloral hydrate(30 mg/kg) and the uterus was removed. The serum estradiol(E2), luteinizing hormone(LH), and follicle stimulating hormone(FSH) were measured by ELISA. The wet mass of bilateral uterus was measured and the coefficient of uterus was calculated. Results: The results of open field experiments showed that the total exercise distance of rats in the Gengshufang high-dose and low-dose groups was longer than that in the model group(P<0.01). The number of rats entering the central area was increased in the estrogen group and the Gengshufang medium-dose group(P<0.05). In addition, the retention time of estrogen group in central area was prolonged(P<0.01). The total distance of exercise in the Gengshufang low-dose group was longer than that in the Kuntai group. The results of the elevated cross maze test showed that the OE% of the model group was lower than that of the control group(P<0.05), the OE% and OT% in the estrogen group, the Gengshufang high-dose and medium-dose group were s

关 键 词：更舒方 围绝经期综合征 卵巢去势 皮温 肛温 血清激素水平 子宫系数 大鼠 

分 类 号：R285.5[医药卫生—中药学]