PER1对人肝癌细胞BEL-7402辐射敏感性的影响  

Effect of PER1 on Radiosensitivity of Human Hepatoma Cell Line BEL-7402

作　　者：潘书贤 肖昌浩 黄皓 胡文涛 PAN Shuxian;XIAO Changhao;HUANG Hao;HU Wentao(State Key Laboratory of Radiation Medicine and Protection,School of Radiation Medicine and Protection,Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions,Soochow University,Suzhou 215123,China)

机构地区：[1]苏州大学医学部放射医学与防护学院放射医学与辐射防护国家重点实验室江苏省高校放射医学协同创新中心

出　　处：《激光生物学报》2020年第1期40-45,53,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(11405235);放射医学与辐射防护国家重点实验室开放课题项目(GZK1201815)

摘　　要：本文通过X射线照射SMMC-7721、BEL-7402和HepG2三种肝癌细胞后,以克隆形成试验检测其存活分数,结果显示在梯度剂量X射线0、2、4、6、8、10 Gy照射下SMMC-7721、BEL-7402、HepG2三种细胞克隆存活分数逐渐下降,其中SMMC-7721在三种肝癌细胞系中对辐射最敏感,BEL-7402辐射抗性在三种肝癌细胞系中最高。Western blot检测发现PER1在SMMC-7721中的表达水平明显显著高于BEL-7402和HepG2(P<0.05)。过表达PER1蛋白以后,BEL-7402接受5 Gy X射线照射后凋亡明显增多,同时,western blot和RT-qPCR试验结果发现,X射线照射过表达PER1的BEL-7402细胞,抗凋亡蛋白Bcl-2表达明显降低,凋亡执行蛋白Caspase-3断裂明显增多。研究结果表明PER1蛋白的高水平表达可以促进X射线诱导的凋亡,增强肝癌细胞的辐射敏感性。In this study,SMMC-7721,BEL-7402 and HepG2 hepatoma cells were irradiated by X-rays,and the survival fraction was detected by colony formation assay;PER1 expression was detected by western blot and RT-qPCR;Flow cytometry was used to detect apoptosis.The results showed that the survival fraction of SMMC-7721,BEL-7402 and HepG2 cells decreased gradually under X-ray irradiation of 0,2,4,6,8,10 Gy,among which SMMC-7721 was the most sensitive one to radiation,while the radioresistance of BEL-7402 was the highest.Western blot analysis showed that the expression level of PER1 in SMMC-7721 was significantly higher than that of BEL-7402 and HepG2(P<0.05).After overexpression of PER1 protein,apoptosis rate of BEL-7402 cells exposed to 5 Gy X-ray irradiation was significantly increased.At the same time,western blot and RT-qPCR showed that expression of anti-apoptotic protein Bcl-2 was significantly decreased,and the cleavage of apoptosis-executing protein caspase-3 was significantly increased in BEL-7402 cells overexpressing PER1.The results showed that high level expression of PER1 protein can promote X-ray induced apoptosis and enhance the radiosensitivity of liver cancer cells.

关 键 词：肝癌 昼夜节律调节蛋白(PER1) 辐射敏感性 放疗 

分 类 号：R81[医药卫生—放射医学]