miR-143在甲状腺乳头状癌中的表达及临床意义  

The expression and clinical significance of miR-143-3p in papillary thyroid cancer

作　　者：郑桂彬[1] 韦树建 吴国长[1] 马驰 孙海清 陈焕杰 林湘峰 赵辉[1] 郑海涛[1] Zheng Guibin;Wei Shujian;Wu Guochang;Ma Chi;Sun Haiqing;Chen Huanjie;Lin Xiangfeng;Zhao Hui;Zheng Haitao(Department of Thyroid Surgery,the Affiliated Yantai Yuhuangding Hospital of Qingdao University,Yantai 264000,China)

机构地区：[1]青岛大学附属烟台毓璜顶医院甲状腺外科,264000

出　　处：《中华内分泌外科杂志》2020年第1期28-31,共4页Chinese Journal of Endocrine Surgery

基　　金：山东省医药卫生科技发展计划(2017WS167)。

摘　　要：目的探究miR-143在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中的表达情况及临床意义。方法收集2018年1月1日至2018年3月31日青岛大学附属烟台毓璜顶医院甲状腺外科52例PTC患者的癌组织标本及癌旁组织标本,采用RT-qPCR分析miR-143在PTC中的表达情况并统计分析miR-143表达与临床病理特点的关系。结果RT-qPCR发现在PTC肿瘤组织中miR-143表达为(3.60±2.98),癌旁正常甲状腺组织中miR-143表达为(20.23±7.09),miR-143表达在PTC癌组织中较癌旁组织中明显降低(t=-21.39,95%CI,18.20~15.07),差异具有统计学意义(P<0.001)。miR-143低表达与PTC患者中央区转移数目≥3(t=10.13,P=0.012)及侧颈部淋巴结转移(t=-4.67,P<0.001)相关。结论miR-143在PTC中表达降低,其表达降低参与PTC淋巴结转移。miR-143可作为PTC治疗中潜在的干预靶点。Objective To explore the expression and clinical significance of miR-143 in papillary thyroid cancer(PTC).Methods Tumor samples and adjacent tissues from 52 patients with PTC were obtained from Jan.1st,2018 to Mar.31st,2018 in Thyroid Surgery Department of the Affiliated Yantai Yuhuangding Hospital of Qingdao University.Quantitative reverse-transcriptase PCR(RT-qPCR)was used to measure the expression of miR-143 in those subjects.In addition,the relationship between the expression levels of miR-143 and the clinicopathological characteristics was analyzed.Results RT-qPCR indicated that the expression of miR-143 was down-regulated in PTC,which was significantly lower than that in adjacent tissues(t=-21.39,95%CI:18.20-15.07,P<0.001).Low expression of miR-143 was related to the number of lymph node metastasis≥3 in central compartment(t=10.13,P=0.012)and lateral neck lymph node metastasis(t=-4.67,P<0.001).Conclusion Downregulation of miR-143 in PTC is linked to the metastasis of PTC and may be a potential target for therapeutic intervention.

关 键 词：MIR-143 甲状腺乳头状癌 淋巴结转移 

分 类 号：R653[医药卫生—外科学] R736.1[医药卫生—临床医学]