肿瘤相关成纤维细胞在甲状腺乳头状癌组织中的表达及其意义  

Priliminary study of the effect of tumor-associated fibroblasts on the infiltration and metastasis of papillary thyroid carcinoma

作　　者：蔡莉[1] 胡金晨[2] 姜蕾[1] 曲桂梅[1] 姜立新[3] 郑海涛[3] Cai Li;Hu Jinchen;Jiang Lei;Qu Guimei;Jiang Lixin;Zheng Haitao(Department of Pathology,the Affiliated Yantai Yuhuangding Hospital of Qingdao University,Yantai 264000,China;Department of Gastrointestinal Surgery,the Affiliated Yantai Yuhuangding Hospital of Qingdao University,Yantai 264000,China;Department of Thyroid Surgery,the Affiliated Yantai Yuhuangding Hospital of Qingdao University,Yantai 264000,China)

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院病理科,264000 [2]青岛大学医学院附属烟台毓璜顶医院胃肠外一科,264000 [3]青岛大学医学院附属烟台毓璜顶医院甲状腺外科,264000

出　　处：《中华内分泌外科杂志》2020年第1期42-46,共5页Chinese Journal of Endocrine Surgery

基　　金：烟台市科技计划项目(2016WS025)。

摘　　要：目的检测甲状腺乳头状癌(papillary thyroid carcinoma,PTC)相关成纤维细胞(tumor-associated fibroblasts,TAFs)中成纤维细胞特异性蛋白-1(fibroblast-specific protein 1,FSP1/S100A4)、平滑肌肌动蛋白-ɑ(ɑ-smooth-muscle actin,ɑ-SMA)和成纤维细胞活化蛋白(fibroblast-activated protein,FAP-ɑ)的表达,探究其与PTC发生、发展的关系。方法通过免疫组化SP法检测FSP1/S100A4、ɑ-SMA、FAP-ɑ在正常甲状腺组织和PTC中的表达情况,并分析这些指标与重要临床病理参数的关系。结果FSP1/S100A4、ɑ-SMA和FAP-ɑ在PTC间质细胞中可见阳性表达,而在正常甲状腺组织的滤泡上皮或间质细胞内均不表达。患者肿瘤的大小、是否有淋巴结转移及TNM的不同分期与FAP-ɑ的阳性病例数有统计学差异(χ^2=6.833,P<0.05;χ^2=10.296,P<0.05;χ^2=4.910,P<0.05);ɑ-SMA阳性病例数在是否被膜侵犯、淋巴结转移有显著差异(χ^2=6.008,P<0.05;χ^2=11.766,P<0.05),而S100A4的阳性病例数在患者的性别、年龄、瘤体的大小、有无被膜侵犯、有无淋巴结转移及患者的TNM分期差异均没有统计学意义(P>0.05)。结论PTC中TAFs的存在可能提示肿瘤具有较强的侵袭转移能力,TAFs有望成为PTC诊疗的一个新靶点。Objective To detect the expression of fibroblast-specific protein 1(FSP1/S100A4),ɑ-smooth-muscle actin(ɑ-SMA)and fibroblast-activated protein(FAP-ɑ)in tumor-associated fibroblasts(TAFs)in papillary thyroid carcinoma(PTC),and to investigate its relationship with the origination and development of PTC.Methods The expression of FSP1/S100A4,ɑ-SMA and FAP-ɑin normal thyroid and PTC was determined by SP method of immunohistochemistry,and the relationship between these indicators and important clinicopathological parameters were analyzed.Results The positive expression of FSP1/S100A4,ɑ-SMA and FAP-ɑwas observed in PTC,but not detected in the follicular epithelium or stromal cells of normal thyroid.In addition,the expression of FAP-ɑwas significantly related to tumor size,lymph node metastasis and TNM classification(χ^2=6.833,P<0.05;χ^2=10.296,P<0.05;χ^2=4.910,P<0.05).The expression ofɑ-SMA was positively related to the invasion of capsule and lymph node metastasis(χ^2=6.008,P<0.05;χ^2=11.766,P<0.05).The expression of FSP1/S100A4 was negatively related to the clinicopathological parameters above(P>0.05)in PTC.Conclusion TAFs in PTC may indicate the infiltration and metastasis,which provideds new thinking for the treatment strategies of papillary thyroid carcinoma.

关 键 词：肿瘤相关成纤维细胞 成纤维细胞特异性蛋白-1 平滑肌肌动蛋白-ɑ 成纤维细胞活化蛋白 甲状腺乳头状癌 

分 类 号：R653[医药卫生—外科学] R736.1[医药卫生—临床医学]