ISSR分子标记技术在植物中的应用及其研究进展  

Application of ISSR Molecular Marker Technology in Plants and Its Research Progress

作　　者：邱国俊[1] 程敏 郭计华[1] QIU Guojun;CHENG Min;GUO Jihua(Xingyi Normal University for Nationalities,Xingyi,Guizhou 562400)

机构地区：[1]兴义民族师范学院,贵州兴义562400

出　　处：《兴义民族师范学院学报》2020年第1期117-120,共4页Journal of Minzu Normal University of Xingyi

基　　金：兴义民族师范学院教授基金项目“贵州山核桃种质资源遗传多样性研究”(项目编号:17XYJS04);贵州省教育厅青年科技人才成长项目“贵州山核桃微卫星(SSR)单核苷酸多态性(SNP)分子标记开发及应用研究”(项目编号:黔教合KY〔2016〕327号)。

摘　　要：简单序列间重复序列(Inter-simple sequence repeat,ISSR)是一种在PCR基础上发展起来的新型分子标记技术。ISSR分子标记具有模板DNA的用量少、操作简单、快速、可靠、多态性丰富等优点。因此有助于鉴定和确定品种遗传多样性的程度,是一种非常理想的遗传标记方法。目前,ISSR分子标记技术在植物上的遗传多样性、鉴定优良种质资源及亲缘关系分析、构建遗传图谱等领域得到了广泛的应用。分析研究ISSR分子标记的原理、特点和方法步骤,及其在植物各领域研究中的应用,以期为今后研究提供参考依据。Inter-simple sequence repeat(ISSR) is a new molecular marker technology developed on the basis of PCR. ISSR molecular markers have the advantages of small amount of template DNA, simple operation, fast, reliable, rich polymorphism and so on. Therefore, it is helpful to identify and determine the degree of genetic diversity of a variety, and is an ideal genetic marker method.At present, ISSR molecular marker technology has been widely used in the fields of plant genetic diversity, identification of excellent germplasm resources, genetic relationship analysis, and construction of genetic maps. This article reviews the principles, characteristics, and method steps of ISSR molecular markers, and their applications in various fields of plants, with a view to providing reference for future research.

关 键 词：ISSR 遗传多样性 种质资源 遗传图谱 

分 类 号：Q94-3[生物学—植物学]