微RNA-155对皮肤间充质干细胞肝细胞生长因子及前列腺素E2基因表达影响的研究  

Influence of miR-155 on HGF and PGE2 gene expression in dermal mesenchymal stem cells

作　　者：侯瑞霞[1] 刘燕敏 赵新程[1] 王方迪 李俊琴[1] 李娟[1] 张开明[1] Hou Ruixia;Liu Yanmin;Zhao Xincheng;Wang Fangdi;Li Junqin;Li Juan;Zhang Kaiming(Institute of Dermatology,Taiyuan Central Hospital,Shanxi Key Laboratory for Immunological Dermatosis,Taiyuan 030009,China)

机构地区：[1]山西医科大学附属太原市中心医院皮肤科免疫性皮肤病干细胞山西省重点实验室

出　　处：《山西医药杂志》2020年第6期651-653,共3页Shanxi Medical Journal

基　　金：国家自然科学基金(81602768);山西省自然科学基金(201801D221441)

摘　　要：目的 研究促炎微RNA(miR)-155对间充质干细胞(DMSCs)肝细胞生长因子(HGF)和前列腺素E2(PGE2)基因表达的影响。方法 采用贴壁法分离DMSCs,分别转染miR-155类似物(mimics)以及抑制物(inhibitors)用以构建高表达或低表达miR-155的DMSC细胞系,采用实时聚合酶链反应(PCR)法比较不同DMSCs HGF和PGE2的表达水平。结果 miR-155 mimics/inhibitor转染可有效地提高或降低DMSCs的miR-155表达水平,高表达miR-155促进HGF,抑制PGE2在DMSCs的表达,而低表达miR-155则抑制HGF,促进PGE2在DMSCs的表达,提示二者均直接或间接受miR-155的调控。结论 miR-155可调节DMSCs的HGF和PGE2表达水平,进而可能对DMSCs的功能产生影响,参与银屑病局部炎症反应的发生和发展。Objective To evaluate the influence of pro-inflammatory microRNA(miR)-155 on gene expression of hepatocyte growth factor(HGF)and prostaglandin E2(PGE2)in dermal mesenchymal stem cells(DMSCs).Methods DMSCs were expanded using adherent assay;miR-155 mimics or inhibitor was transfected into DMSCs to conduct a miR-155 over-expressing or low-expressing cell model.Real-time PCR was used to detect the gene expression of HGF and PGE2.Results miR-155 mimics/inhibitor can efficiently increase or inhibit the miR-155 expression in DMSCs,respectively.Over-expression of miR-155 induce elevated HGF and declined PGE2 expression,while low-expression of miR-155 inhibit HGF and promote PGE2 expression.Conclusion miR-155 can regulate the expression of HGF and PGE2,which may further influence the biological function of DMSCs and then participate in the pathogenesis of psoriasis.

关 键 词：银屑病 间质干细胞 微RNAS 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]