斧翅沙芥蔗糖转运蛋白基因PdSUT4的克隆与表达分析  

Cloning and expression analysis of a sucrose transporter gene in Pugionium dolabratum

作　　者：杨宁[1] 张莉环 史万斌 刘博 王玮[1] YANG Ning;ZHANG Li-huan;SHI Wan-bin;LIU Bo;WANG Wei(College of Life Science,Northwest Normal University,Lanzhou 730070,Gansu,Chiana;Xujiashan Forest Farm,Lanzhou 730070,Gansu,China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]徐家山林场,甘肃兰州730046

出　　处：《西北师范大学学报（自然科学版）》2020年第2期87-93,共7页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31660116)。

摘　　要：通过5′和3′cDNA末端快速扩增方法获得PdSUT4基因序列,利用生物信息学软件预测蛋白质理化性质、结构域和亚细胞定位等分子特性,同时用实时荧光定量PCR(qRT-PCR)进行PdSUT4基因表达模式分析.结果表明,从斧翅沙芥克隆得到的PdSUT4基因cDNA全长为2005 bp,开放阅读框1536 bp,编码511氨基酸,相对分子质量为55087.44,等电点为9.26,PdSUT4蛋白含有12个跨膜结构域,具有高等植物蔗糖/H+共转运家族和协助扩散超家族的保守结构域,与多种植物SUT4基因编码的蛋白有较高的一致性;干旱胁迫下斧翅沙芥PdSUT4基因表达量迅速升高.PdSUT4基因的克隆与表达分析为进一步研究该基因在斧翅沙芥生长发育、逆境生理的生物学功能奠定了基础.PdSUT4 gene sequence was obtained by rapid amplification of 5′and 3′cDNA ends.Characteristics of physiochemical properties,conserved domains and subcellular localization of the deduced PdSUT4 protein were determined by using a series of bioinformatics tools.Gene expression patterns were performed by real time quantitative PCR.The results showed that PdSUT4 is cloned and its full length is 2005 bp.The open reading frame is 1536 bp,encoding 511 amino acids with a relative molecular mass of 55087.44 Da and an isoelectric point of 9.26.The deduced PdSUT4 protein contains 12 transmembrane domains,which are conserved domains of sucrose/H+symporter and diffusion-assisted superfamily in higher plants.The PdSUT4 protein was highly consistent with the protein encoded by various plant SUT4 genes.Drought stress induced the expression of PdSUT4 gene significantly.The data presented here laid a foundation for further study on the gene involving in the growth,development and stress physiology of P.dolabratum.

关 键 词：斧翅沙芥 蔗糖转运蛋白 生物信息学分析 干旱胁迫 基因表达 

分 类 号：S566.1[农业科学—作物学]