磺基水杨酸和精氨酸构筑的Cu(Ⅱ)配合物与DNA的相互作用  

Interaction between DNA and Cu(Ⅱ)Complex Constructed by 5-Sulfosalicylic Acid and L-Arg

作　　者：李小芳 冯小强 朱元成 王彦博 LI Xiaofang;FENG Xiaoqiang;ZHU Yuancheng;WANG Yanbo(School of Chemical Engineering and Technology,Tianshui Normal University,Tianshui 741001,Gansu Province,China)

机构地区：[1]天水师范学院化学工程与技术学院,甘肃天水741001

出　　处：《吉林大学学报（理学版）》2020年第2期422-427,共6页Journal of Jilin University:Science Edition

基　　金：甘肃省青年科技基金(批准号:1606RJYE247);天水师范学院创新能力提升项目(批准号:CXT2019-52).

摘　　要：首先,合成并表征一种新型配合物Cu2(L-Arg)2(SSA)(H2O)2(L-Arg=精氨酸,SSA=磺基水杨酸),然后以鲱鱼精DNA为靶点,用电化学法、光谱法和黏度法研究配合物与DNA间的相互作用。结果表明:当扫描速率为0.1V/s时,配合物在0.1248,-0.5189V处出现一对明显的氧化还原峰,且配合物的氧化还原峰电流随扫描速率的增大而增大,配合物在玻碳电极上的反应由扩散过程控制;加入DNA后,配合物的氧化和还原峰电流均减小,式量电位增大,配合物的峰位发生移动,吸光强度降低,且与DNA的浓度呈正相关;配合物的相对黏度呈增大趋势。因此配合物与DNA间存在嵌插作用。We synthesized and characterized a new complex of Cu2(L-Arg)2(SSA)(H2O)2(L-Arg=L-Arginine,SSA=5-sulfosalicylic acid),and studied interaction between complex and DNA by electrochemical method,spectroscopic method and viscosity method with herring sperm DNA as bimolecular target.The results show that when the scanning rate is 0.1 V/s,apair of obvious redox peaks appear at 0.1248,-0.5189 V,the redox peak current of the complex increases with the scanning rate,and the reaction of complex at glassy carbon electrodes is controlled by diffusion process.After adding DNA,the oxidation and reduction peak currents of the complex decrease,the formal potential increases,the peak position of the complex shifts,the absorption intensity decreases,which is positively related to the concentration of DNA,and the relative viscosity of the complex increases.Therefore,there is intercalation between complex and DNA.

关 键 词：精氨酸 磺基水杨酸 铜(Ⅱ) 鲱鱼精DNA 

分 类 号：O629.74[理学—有机化学]