Impact of anthropogenic activities on the sediment microbial communities of Baiyangdian shallow lake  

作　　者：Wenjun Wang Yujun Yi Yufeng Yang Yang Zhou Shanghong Zhang Xuan Wang Zhifeng Yang 

机构地区：[1]State Key Laboratory of Water Environmental Simulation,School of Environment,Beijing Normal University,Beijing,100875,China [2]Ministry of Education Key Laboratory of Water and Sediment Science,School of Environment,Beijing Normal University,Beijing 100875,China [3]Renewable Energy School,North China Electric Power University,Beijing 102206,China

出　　处：《International Journal of Sediment Research》2020年第2期180-192,共13页国际泥沙研究（英文版）

基　　金：This study is supported by the National Natural Science Foundation of China(51439001,51722901);the National Key R&D Program of China(2018YFC0407403);the Fundamental Research Funds for the Central Universities;the Interdiscipline Research Funds of Beijing Normal University。

摘　　要：Microorganisms are recognized as sensors of external disturbance in freshwater ecosystems.In this study,sediment samples from regions with strong human activity interference(four sites),moderate human activity interference(five sites)and minor human activity interference(five sites)in Baiyangdian Lake were collected;and their physiological and chemical properties and heavy metals contents were analyzed.The structures of bacterial and fungal communities were tested using 16S ribosomal Ribonucleic Acid(rRNA)and Internal Transcribed Spacer 2(ITS2)high-throughput sequencing technologies.The results indicated that Proteobacteria was the most abundant bacterial phylum.Ascomycota,Basidiomycota and Ciliophora were the dominant phyla of fungi.Obvious differences of microbial genera among the three regions were observed,especially fungal genera.With intensified disturbance by human activities,the Simpson and Shannon indices of bacteria significantly decreased.The fungal communities were clustered into three groups in accordance with the different human disturbance levels.The bacterial communities of sediment samples under moderate and minor human disturbance appeared similar,but different from those under strong human disturbance.Redundancy analysis indicated that zinc,total phosphorus,copper,and lead were the dominant factors that influenced the bacterial community.Nitrifiers and sulfate-reducing bacteria were influenced by pH,nitrate(NO3 ),and total nitrogen.The fungal community was significantly affected by total phosphorus.This study reveals that sediment microbial community structures among different regions of a shallow lake are mainly impacted by human activities.Microorganisms are recognized as sensors of external disturbance in freshwater ecosystems.In this study,sediment samples from regions with strong human activity interference(four sites),moderate human activity interference(five sites) and minor human activity interference(five sites) in Baiyangdian Lake were collected;and their physiological and chemical properties and heavy metals contents were analyzed.The structures of bacterial and fungal communities were tested using 16 S ribosomal Ribonucleic Acid(rRNA) and Internal Transcribed Spacer 2(ITS2) high-throughput sequencing technologies.The results indicated that Proteobacteria was the most abundant bacterial phylum.Ascomycota,Basidiomycota and Ciliophora were the dominant phyla of fungi.Obvious differences of microbial genera among the three regions we re obse rved,especially fungal genera.With intensified disturbance by human activities,the Simpson and Shannon indices of bacteria significantly decreased.The fungal communities were clustered into three groups in accordance with the different human disturbance levels.The bacterial communities of sediment samples under moderate and minor human disturbance appeared similar,but different from those under strong human disturbance.Redundancy analysis indicated that zinc,total phosphorus,copper,and lead were the dominant factors that influenced the bacterial community.Nitrifiers and sulfate-reducing bacteria were influenced by pH,nitrate(NO3),and total nitrogen.The fungal community was significantly affected by total phosphorus.This study reveals that sediment microbial community structures among different regions of a shallow lake are mainly impacted by human activities.

关 键 词：BACTERIAL COMMUNITY FUNGAL COMMUNITY Human activity INTERFERENCE Environmental factors 

分 类 号：Q938[生物学—微生物学]