两种类型标本糖化清蛋白检测结果的评估  

Evaluation on detection results of glycated albumin in two types of specimens

作　　者：杨浩[1] 付靖瑜 尹义军[1] 汪宏良[1] YANG Hao;FU Jingyu;YIN Yijun;WANG Hongliang(Department of Clinical Laboratory,Huangshi Municipal Central Hospital,Edong Healthcare Group/Affiliated Hospital of Hubei Polytechnic University,Huangshi,Hubei 435000,China)

机构地区：[1]鄂东医疗集团黄石市中心医院/湖北理工学院附属医院医学检验科,湖北黄石435000

出　　处：《检验医学与临床》2020年第6期748-750,共3页Laboratory Medicine and Clinic

基　　金：湖北省卫生健康委员会科研基金项目(WJ2019M044)。

摘　　要：目的评估静脉血浆和血清标本糖化清蛋白(GA)检测结果之间的差异。方法选取2019年1月于该院就诊的患者75例,无特定的排除标准,收集其乙二胺四乙酸二钾(EDTA-K 2)抗凝静脉血和无添加剂静脉血标本各1管,分离出血浆和血清,检测清蛋白(ALB)、GA(%)水平,应用统计学方法分析分别使用血浆标本和血清标本检测GA(%)的偏倚。结果ALB水平为26.3~47.5 g/L,GA(%)水平为10.5%~20.0%,静脉血浆与血清的ALB水平差异有统计学意义(P<0.05),GA(%)水平差异无统计学意义(P>0.05)。呈偏态分布的血浆与血清GA(%)相对偏差的中位数为-1.01%,Passing-Bablok回归模型拟合得到的回归方程为Y=-1.882+1.126 X,将GA(%)诊断切点14.3%和16.3%代入回归方程,偏倚分别为-0.15%和1.41%,均小于生物学变异研究的预期偏倚范围(±2.9%)。结论GA(%)的水平在10.5%~20.0%时,EDTA-K 2抗凝静脉血浆与无添加剂静脉血清标本用于GA检测的差异小,其偏倚在临床可接受范围内,均可在临床使用。Objective To evaluate the difference in the detection results of glycated albumin between venous plasma and serum samples.Methods EDTA-K 2 anticoagulant venous blood and no additive venous blood were collected from 75 patients treated in the hospital during January 2019 were selected.Each tube of anti-coagulation venous blood by EDTA-K 2 and venous blood without additive was collected for separating plasma and serum.The levels of albumin(ALB)and glycated albumin GA(%)were detected.The bias of GA(%)detected by using the plasma and serum samples was analyzed by using the statistical method.Results The ALB levels ranged 26.3-47.5 g/L,the GA(%)levels ranged 10.5%-20.0%,the ALB difference between venous plasma and serum was statistically significant(P<0.05),the difference in GA(%)had no statistical significance(P<0.05).The relative bias of GA(%)of plasma and serum showing the skewed distribution had the median of-1.01%.The regression equation obtained by the Passing-Bablok regression model fitting was Y=-1.882+1.126 X,the GA(%)diagnostic cut points of 14.3% and 16.3% were substituted into the regression equation,the biases were-0.15% and 1.41% respectively,which all were less the expectation bias range(±2.9%)in biologic variation study.Conclusion When the GA(%)level is in 10.5%-20.0%,the difference is little in the GA detection by using the EDTA-K 2 anti-coagulation plasma sample and serum sample without additive,their bias is acceptable in clinic and all can be used in clinic.

关 键 词：糖化清蛋白 偏倚 生物学变异 糖尿病 

分 类 号：R446.11[医药卫生—诊断学]