利用马克隆值差异显著的BILs鉴定棉纤维品质相关基因  

Identification of Differentially Expressed Genes in Developing Cotton Fibers between Two Groups of Backcross Inbred Lines Differing in Fiber Micronaire

作　　者：吴嫚[1] 李龙云[1] 裴文锋[1] Zhang Jinfa 于霁雯[1] Wu Man;Li Longyun;Pei Wenfeng;Zhang Jinfa;Yu Jiwen(Zhengzhou Research Base,State Key Laboratory of Cotton Biology,Zhengzhou University,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,Henan,China;Department of Plant and Environment Sciences,New Mexico State University,Las Cruces 880033,USA)

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室郑州大学研究基地,河南安阳455000 [2]Department of Plant and Environmental Science,New Mexico State University,Las Cruces,NM88003,USA

出　　处：《棉花学报》2020年第1期52-62,共11页Cotton Science

基　　金：国家重点研发计划(2018YFD0100300)。

摘　　要：【目的】马克隆值是衡量棉纤维品质的重要指标之一。利用马克隆值存在显著差异的2个陆海回交近交系材料进行高通量基因芯片分析,旨在筛选和鉴定棉纤维发育相关的关键基因。【方法】从SG747(Gossypium hirsutum L.)和Giza75(Gossypium Barbadense L.)高世代回交近交系(Backcross Inbred Lines,BILs)群体中选取NMGA-140(马克隆值6.2)和NMGA-051(马克隆值4.0)为材料,利用Affymetrix公司的棉花寡聚核苷酸基因芯片对其开花后10 DPA(Days after anthesis)的棉纤维进行了表达谱分析。【结果】获得了2655个差异表达基因,包括功能预测基因(15.57%),翻译、核糖体结构与生物合成相关基因(13.54%)和翻译后修饰、蛋白质转换、分子伴侣相关基因(9.31%)等。推测β-tub10正向调控棉纤维发育过程,β-tub1负向调控棉纤维发育过程,Ghi.3578.1.S1_s_at在棉纤维发育早期起到了作用。【结论】为棉花纤维品质的遗传改良提供了丰富的基因资源,为分子标记辅助育种开发更多新的分子标记。[Objective]The micronaire is one of the important indicators affecting the quality of cotton fiber.High throughput microarray analysis of two upland cotton/sea-island cotton backcross inbred lines with significant differences in micronaire values was designed to screen and identify key genes involved in cotton fiber development.[Method]The two BIL lines,NMGA-140 and NMGA-051 were selected from a backcross inbred lines(BILs),which were developed form a cross between upland cotton SG747(Gossypium hirsutum L.)and Egyptian cotton Giza75(G.barbadense L.).The Affymetrix Cotton GeneChip was then used to perform a transcriptome analysis in developing fibers(10 Days post anthesis,DPA).[Result]There are 2655 transcripts showed 2-fold or higher levels of expression changes between the two BIL lines.Clustering analysis of these DE genes by Clusters of Orthologous Groups database was performed,and revealed that many genes were related to general function prediction only(15.57%),translation,ribosomal structure and biogenesis(13.54%),posttranslational modification,protein turnover,chaperones(9.31%).The quantitative real time polymerase chain reaction(qRT-PCR)results indicated thatβ-tub1,β-tub10,and Ghi.3578.1.S1_s_at were highly expressed in the periods of fast fiber elongation and cell wall synthesis(5-25 DPA),implicating their roles in fiber micronaire development.[Conclusion]It provides a list of putative candidate genes for further studies in identifying genes responsible for fiber traits and developing molecular markers for marker-assisted breeding.

关 键 词：棉纤维品质 马克隆值 基因芯片 差异表达基因 

分 类 号：S562[农业科学—作物学]