蓖麻eIF5A基因克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of Castor eIF5A Gene

作　　者：赵慧博 李丽丽 梁塔娜 张艳欣 黄凤兰[1,2] 曹清国 Zhao Huibo;Li Lili;Liang Tana;Zhang Yanxin;Huang Fenglan;Cao Qingguo(College of Life Science,Inner Mongolia University for the Nationalities,Tongliao,028000;Inner Mongolia Industrial Engineering Research Center of University for Castor,Inner Mongolia Key Laboratory of Castor Breeding,Inner Mongolia Collaborative Innovation Center for Castor Industry,Inner Mongolia University for the Nationalities,Tongliao,028000;Animal Husbandry and Veterinary Grassland Station,Wuerqihan Town,Yakeshi City,Yakeshi,022159)

机构地区：[1]内蒙古民族大学生命科学学院,通辽028000 [2]内蒙古民族大学,内蒙古自治区高校蓖麻产业工程技术中心,内蒙古自治区蓖麻育种重点实验室,内蒙古自治区蓖麻产业协同创新中心,通辽028000 [3]内蒙古牙克石市乌尔其汉镇畜牧兽医草原站,牙克石022159

出　　处：《分子植物育种》2020年第4期1103-1108,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31860071);内蒙古自治区自然科学基金面上项目(2017MS0339);内蒙古自治区草原英才创新团队——蓖麻分子育种创新研究团队支持项目(2017);内蒙古民族大学自治区科技储备项目子课题(2018ND-CB05-2);内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目(MDK2018014)共同资助。

摘　　要：真核细胞翻译起始因子5A(eIF5A)是一种广泛存在真菌、动植物体内的蛋白质翻译起始因子,也是一种对调控生物生长发育、衰老及环境适应等产生重要作用的影响因子。本研究利用设计的蓖麻蛋白翻译起始因子5A基因的引物对蓖麻‘2129’基因组DNA进行PCR扩增并进行生物信息学分析。结果发现,该目的片段长为480bp,通过对该基因进行理化性质分析发现其分子量为39670.20Da,等电点(pi)为5.22,是一种疏水性不稳定蛋白,通过多序列比对和系统发育树分析发现,蓖麻eIF5A与同属大戟科的麻疯树亲缘关系最近,而与锦葵科的陆地棉亲缘关系最远。以上结果将为今后揭示蓖麻eIF5A蛋白功能提供重要的线索。Eukaryotic translation initiation factor 5A(eIF5A)is a protein translation initiation factor widely present in firngi,animals and plants,and is an influencing factor that plays an important role in regulating growth,development,aging and environmental adaptation.In this paper,the castor'2129'genomic DNA was amplified by PCR and bioinformatics analysis using the primers of the designed ricin translation initiation factor 5A gene.The result showed that the target fragment was 480 bp.The molecular weight of the gene was found to be 39670.20 Da and the isoelectric point(pi)was 5.22.It was a hydrophobic unstable protein and passed multiple sequence alignment and system.The developmental tree analysis found that castor eIF5A has the closest relationship with Jatropha curcas in the same Euphorbiaceae family,and has the closest relationship with the cotton of the Malvaceae.These results will provide important clues for revealing the function of ramie eIF5A protein in the future.

关 键 词：蓖麻 真核翻译起始因子 基因克隆 生物信息学分析 

分 类 号：S565.6[农业科学—作物学]