水稻闭颖授粉QTL的初步定位分析  

Analysis of the Preliminary Mapping QTL for Cleistogamy in Rice

作　　者：周师旭 林晓娜 陈荣杨 苏春林 李容柏[2,3] 罗继景[1,2] 岑卫健[2] Zhou Shixu;Lin Xiaona;Chen Rongyang;Su Chunlin;Li Rongbai;Luo Jijing;Cen Weijian(College of Life Science and Technology,Guangxi University,Nanning,530004;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangxi University,Nanning,530004;College of Agriculture,Guangxi University,Nanning,530004)

机构地区：[1]广西大学生命科学与技术学院,南宁530004 [2]广西大学,亚热带农业生物资源保护与利用国家重点实验室,南宁530004 [3]广西大学农学院,南宁530004

出　　处：《分子植物育种》2020年第4期1187-1192,共6页Molecular Plant Breeding

基　　金：广西高校引进海外高层次才‘百计划’专项经费(2015);广西研究生教育创新计划项目(YCBZ2017012)共同资助。

摘　　要：水稻闭颖授粉遗传位点的发掘旨在研究其闭颖授粉机制。本研究利用闭颖水稻品系RV2429为母本,开颖授粉水稻品系Koshihikari为父本杂交得到F1代,F1自交得到F2,以双亲本及F2为研究材料,对双亲本的粒型及花器官结构进行比较,推测出浆片体积增加较小是RV2429闭颖授粉的原因。统计F2群体的开花表型和基因型,通过QTL IciMapping 4.1软件分析,初步定位到4个与闭颖授粉相关的QTLs,qCL4.1(4.75%),LOD值为2.559 3、qCL6.1 (5.12%),LOD值为2.999 1、qCL11.1 (5.06%),LOD值为2.799 6和qCL12.1(4.77%),LOD值为3.052 1,分别在4、6、11、12染色体上。本研究结果明确了控制水稻闭颖授粉的新基因位置,为以后的精细定位提供了实验依据,为加快闭颖授粉的育种进程提供帮助,对抑制基因漂流具有重要意义。The genetic mapping QTL for cleistogamy in rice aims at studying its cleistogamy mechanism. In this study, a F2 population was constructed by crossing between RV2429, a closed pollinated variety, and Koshihikari,the open pollinated rice, and was used for cleistogamy QTL mapping. By comparison with Koshihikari, the florets with a pair of small lodicules in RV2429 may result in failing to open the glumes. Four QTLs were detected on chromosome 4, 6, 11, and 12 by genetic linkage analysis of QTL IciMapping 4.1 molecular marker mapping software. These QTLS were qCL4.1(4.75%), LOD 2.559 3, qCL6.1(5.12%), LOD 2.999 1, qCL11.1(5.06), LOD2.799 6 and q CL12.1(4.77%), LOD 3.052 1. The results of this study figure out the new QTLs of cleistogamy, will provide experimental basis for further fining mapping, offer help to accelerate the breeding process of closed cleistogamy, and have important significance for inhibiting gene drift.

关 键 词：水稻 闭颖授粉 QTL定位 遗传分析 

分 类 号：S511[农业科学—作物学]