微流控芯片非接触电导法快速测定盐酸美金刚片中盐酸美金刚的含量  

Rapid Determination of Memantine Hydrochloride in Memantine Hydrochloride Tablets by Contactless Conductivity Method with Microfluidic Chip

作　　者：李智磊 李静岚 陈缵光[3] 王宇航 胡姗姗 杨秀娟[1] 王勇[1] LI Zhi-lei;LI Jing-lan;CHEN Zuan-guang;WANG Yu-hang;HU Shan-shan;YANG Xiu-juan;WANG Yong(Department of Pharmacy,ZhuJiang Hospital of Southern Medical University,Guangzhou 510280,China;Department of Pharmacy,Shenzhen Second People s Hospital,Shenzhen 518035,China;School of Pharmacy,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]南方医科大学珠江医院药学部,广东广州510280 [2]深圳市第二人民医院药学部,广东深圳518035 [3]中山大学药学院,广东广州510006

出　　处：《分析测试学报》2020年第2期263-267,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(81302738)。

摘　　要：建立了微流控芯片非接触电导法快速测定盐酸美金刚片中盐酸美金刚含量的方法。探讨了缓冲溶液的种类、浓度、pH值、进样时间及分离电压等因素对分离检测的影响。优化得到缓冲体系为含2%(体积分数)二甲基亚砜(DMSO)的3.0 mmol/L三乙胺-2.0 mmol/L磷酸缓冲溶液(pH3.3),进样时间为10 s,分离电压为2.0 kV。优化条件下,盐酸美金刚的线性范围为10~2000μg/mL(r^2=0.9991);检出限为7μg/mL,定量下限为10μg/mL;精密度、稳定性、重复性实验的相对标准偏差(RSD)均小于2.0%;平均加标回收率为96.5%~99.2%;盐酸美金刚的检测时间小于18 s。该方法快速简便,适用于盐酸美金刚片中盐酸美金刚含量的测定。A contactless conductivity method with microfluidic chip was established for the rapid determination of memantine hydrochloride in memantine hydrochloride tablet.Effects of electrophoresis parameters such as variety,concentration,pH value of buffer solution,injection time and separation voltage on the separation detection were investigated.The optimized buffer system was 3.0 mmol/L triethylamine-2.0 mmol/L phosphate buffer solution(pH 3.3)containing 2%(by volume)dimethyl sulfoxide(DMSO),the injection time was 10 s and the separation voltage was 2.0 kV.In the optimal conditions,the linear range for memantine hydrochloride was 10-2000μg/mL(r^2=0.9991).The limits of detection and quantitation were 7μg/mL and 10μg/mL,respectively.Relative standard deviations for precision,stability and reproducibility tests were lower than 2.0%,and average recoveries for memantine hydrochloride were in the range of 96.5%-99.2%,with a detection time less than 18 s.The method is rapid and simple,and is suitable for the determination of memantine hydrochloride in memantine hydrochloride tablet.

关 键 词：微流控芯片 非接触电导检测 盐酸美金刚 盐酸美金刚片 

分 类 号：O657.11[理学—分析化学] R914.1[理学—化学]