祛乳癖胶囊挥发性部位的研究  

Research on the Volatile Part of Qurupi Capsules(祛乳癖胶囊)

作　　者：任慧[1] 陈志永 陈娟 孟雪[1] 杜霞[1] 郭冬[1] REN Hui;CHEN Zhi-yong;CHEN Juan;MENG Xue;DU Xia;GUO Dong(Shaanxi Academy of Traditional Chinese Medicine,Xi'an Shaanxi 710003,China)

机构地区：[1]陕西省中医药研究院,陕西西安710003

出　　处：《中医药导报》2020年第2期20-24,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省社会发展科技攻关项目(2016SF-398)。

摘　　要：目的:建立祛乳癖胶囊挥发油的提取、包合工艺及包合物质量标准。方法:采用正交试验优选祛乳癖胶囊挥发油的提取及包合工艺,并借助薄层色谱法(TLC)对包合物中的当归、香附进行定性鉴别,采用高效液相色谱法(HPLC)测定包合物中藁本内酯的含量。结果:挥发油最佳提取工艺为8倍量水,浸泡2 h,提取9 h;最佳包合工艺是50%挥发油乙醇溶液∶β-环糊精水溶液(v/v)为1.6∶50,在45℃包合1 h;建立了包合物的定性鉴别方法和藁本内酯的含量测定方法。结论:提取、包合工艺及质量控制方法合理可行,为祛乳癖胶囊的制备工艺及质量控制奠定了基础。Objective: To study on the extraction method and inclusion process of the volatile oil of Qurupi capsules, and explore the quality standard of the inclusion complex. Methods: The orthogonal design was used to investigate the extraction method and inclusion process of the volatile oil of Qurupi Capsules. TLC was used for qualitative identification of Danggui(Angelica sinensis) and Xiangfu(Cyperus rotundus). HPLC method was adopted to determine the contents of ligustilide. Results: The extraction procedure: 8 times water, soak for 2 hours and extraction 9 h. The inclusion procedure: 50% volatile oil ethanol solution:β-cyclodextrins aqueous solution was 1.6∶50, the inclusion temperature was 45 ℃ and the inclusion time was 1 hour. The methods of qualitative identification and content determination were established. Conclusions: The extraction method, inclusion process and quality control method are reasonable and feasible, and lays a foundation for the formation technology of Qurupi capsules and its quality standards.

关 键 词：祛乳癖胶囊 挥发油 提取工艺 包合工艺 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]