白藜芦醇对肾癌细胞体外增殖与侵袭能力的影响  

Resveratrol inhibits the proliferation and invasion of renal cell carcinoma cells in vitro

作　　者：张栋[1] 杨小杰[1] 雒启东[1] 付德来[1] 李钊伦[1] 张鹏[1] 种铁[1] ZHANG Dong;YANG Xiao-jie;LUO Qi-dong;FU De-lai;LI Zhao-lun;ZHANG Peng;CHONG Tie(Department of Urology,Second Hospital of Xi'an Jiaotong University,Xi'an 710004,China)

机构地区：[1]西安交通大学第二附属医院泌尿外科,陕西西安710004

出　　处：《现代泌尿外科杂志》2020年第3期258-261,共4页Journal of Modern Urology

基　　金：陕西省自然科学基础研究计划项目(No.2018JM7093);西安交通大学自由探索与创新项目(No.XJJ2018138)。

摘　　要：目的探讨白藜芦醇对肾癌786-O与ACHN细胞体外迁增殖与侵袭能力的影响及可能的机制。方法 MTT检测白藜芦醇对肾癌细胞增殖能力的影响,划痕实验检测白藜芦醇对786-O与ACHN细胞体外迁移能力的影响,Transwell实验检测白藜芦醇对786-O与ACHN细胞体外侵袭能力的影响,RT-PCR与Western bolt检测白藜芦醇对MMP-2与MMP-9蛋白表达水平的影响。结果白藜芦醇可抑制膀胱癌细胞体外生长能力,且呈剂量依赖性。30μmol/L白藜芦醇处理786-O与ACHN细胞24h后,划痕实验发现白藜芦醇可抑制786-O与ACHN细胞体外迁移能力,Transwell实验证实白藜芦醇可抑制肾癌细胞786-O与ACHN的体外侵袭能力。RT-PCR与Western bolt结果表明,白藜芦醇可从mRNA与蛋白水平抑制MMP-2与MMP-9的表达。结论白藜芦醇能抑制肾癌细胞体外增殖能力,可能通过下调MMP-2与MMP-9的表达而抑制肾癌细胞786-O与ACHN体外迁移与侵袭能力,有望成为治疗肾癌的新策略。Objective To investigate effects of resveratrol on the proliferation and invasion ability of renal cell carcinoma(RCC) 786-0 and ACHN cells in vitro and the possible mechanism.Methods Effects of resveratrol on the proliferation,migration and invasion of 786-0 and ACHN cells were determined with MTT,wound healing test and Transwell assay,respectively.Effects of resveratrol on the expressions of MMP-2 and MMP-9 were detected by real-time PCR and Western blot.Results Resveratrol inhibited the proliferation of RCC cells in a dose-dependent manner.After 786-0 and ACHN cells were treated with 30 μmol/L resveratrol for 24 h,their migration and invasive capability were inhibited.Resveratrol decreased the mRNA and protein expressions of MMP-2 and MMP-9.Conclusion Resveratrol can inhibit the proliferation and invasion of RCC 786-0 and ACHN cells by downregulating the expressions of MMP-2 and MMP-9.Resveratrol may be a promising treatment for RCC.

关 键 词：白藜芦醇 肾细胞癌 MMP-2 MMP-9 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]