功能化石墨烯对海洋环境中细菌群落结构及生长的影响  

Effects of functionalized graphene on bacterial community structure and growth in marine environments

作　　者：张作峰 曾令宇 王兆守[1,2] 邵宗泽 ZHANG Zuofeng;ZENG Lingyu;WANG Zhaoshou;SHAO Zongze(College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;Key Laboratory of Marine Biological Genetic Resources,Ministry of Natural Resources,The Third Institute of Oceanography,Ministry of Natural Resources of China,Xiamen 361005,China)

机构地区：[1]厦门大学化学化工学院,福建厦门361005 [2]自然资源部第三海洋研究所,自然资源部海洋生物遗传资源重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2020年第2期271-277,共7页Journal of Xiamen University：Natural Science

基　　金：国家微生物资源平台项目(NIMR-2019-9);厦门市海洋经济创新发展示范项目(16PZP001SF16);国家海洋局海洋生物遗传资源重点实验室开放研究基金(HY201505)。

摘　　要：功能化石墨烯因其优异的导电、传热和机械性能,应用前景广阔.为了解功能化石墨烯对海洋环境可能的生态毒性效应,其分别加入近岸海水(终质量浓度50 mg/L)和沉积物(终质量分数50 mg/kg)中,模拟海洋环境对样品培养2周,并采用DNA高通量测序技术分析功能化石墨烯对海洋细菌群落结构的影响.结果表明功能化石墨烯对两种海洋环境来源的细菌物种组成均有明显影响,但效果不同:暴露于功能化石墨烯后,海水中细菌的物种多样性和丰度降低,而沉积物中细菌的物种多样性和丰度升高,这可能是功能化石墨烯在海水和海洋沉积物中分散、吸附和团聚等环境行为存在差异所致.然而,在两种海洋环境来源的处理组中,功能化石墨烯的存在均对光合细菌(蓝细菌门)的生长起促进作用.上述结果可为功能化石墨烯的海洋生态安全性评估提供参考.Functionalized graphene has broad application due to its excellent electrical conductivity,heat transfer and mechanical properties.In order to explore the possible ecotoxic effects of functionalized graphene on marine environments,we added functionalized graphene into coastal seawater(50 mg/L)and sediments(50 mg/kg)respectively,and cultured the samples for 2 weeks under conditions that simulated the marine environments.The effect of functionalized graphene on the structure of marine bacterial community was analyzed by high-throughput DNA sequencing technology.The results showed that functionalized graphene had significant but different effects on the species composition of bacteria from two different marine environments.Upon exposure to functionalized graphene,species diversity and abundance of bacteria in seawater reduced,while species diversity and abundance of bacteria in sediments increased.This may be due to the differences in the environmental behaviors such as dispersion,adsorption and aggregation of functionalized graphene in seawater and marine sediments.However,functionalized graphene promoted the growth of photosynthetic bacteria(Cyanobacteria)in both seawater and marine sediments.This study provides a reference for the assessment of marine ecological safety of functionalized graphene.

关 键 词：功能化石墨烯 海洋细菌 群落结构 多样性分析 

分 类 号：Q938.1[生物学—微生物学]