花姜酮对Hela细胞株细胞生物学特性的影响  

Effects of zingiberenone on proliferation,migration,invasion and apoptosis of HeLa cell line

作　　者：吴玲姣[1] 邓晓杨[1] 徐蕾[1] 邓洁[1] 朱晓莺[1] 吴海燕[1] WU Lingjiao;DENG Xiaoyang;XU Lei;DENG Jie;ZHU Xiaoying;WU Haiyan(Department of Gynaecology,The First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院第一附属医院妇科,四川成都610500

出　　处：《西部医学》2020年第3期365-368,378,共5页Medical Journal of West China

基　　金：四川省教育厅资助课题重点项目(17ZA0128)。

摘　　要：目的探究花姜酮对Hela细胞株增殖、迁移、侵袭及凋亡的影响。方法购买人宫颈癌Hela细胞株,进行培养并分为空白组、花姜酮5μmol/L组、花姜酮10μmol/L组、花姜酮20μmol/L组4组。空白组使用常规细胞培养液培养,其他三组分别使用相对应浓度的花姜酮进行培养。观察4组细胞的增殖、迁移、侵袭、凋亡情况及上皮细胞间充质(EMT)相关蛋白相对表达量。结果24、48、72 h花姜酮20μmol/L组细胞增殖率低于花姜酮5μmol/L组、花姜酮10μmol/L组及空白组(P<0.05);24、48、72 h花姜酮20μmol/L组细胞凋亡率高于花姜酮5μmol/L组、花姜酮10μmol/L组及空白组(P<0.05);花姜酮20μmol/L组细胞迁移、侵袭数均低于花姜酮5μmol/L组、花姜酮10μmol/L组及空白组(P<0.05);花姜酮20μmol/L组细胞波形蛋白(Vimentin)、组蛋白甲基转移酶同源序列2增强子(EZH2)相对表达量均低于花姜酮5μmol/L组、花姜酮10μmol/L组空白组;花姜酮20μmol/L组细胞α-连环蛋白(α-cat)、上皮性钙粘蛋白(E-cadherin)相对表达量均高于花姜酮5μmol/L组、花姜酮10μmol/L组及空白组(P<0.05)。结论花姜酮能够促进HeLa细胞株的凋亡,抑制Hela细胞株的增殖、迁移及侵袭能力,其能力呈现浓度依赖。Objective To investigate the effects of zingiberenone on proliferation,migration,invasion and apoptosis of HeLa cell line.Methods HeLa cell line of human cervical cancer was cultured and divided into blank group,5-μmol/L group,10-μmol/L group and 20-μmol/L group.Cell proliferation,migration,invasion,apoptosis and relative expression of EMT-related proteins were observed.Results At 24 h,48 h and 72 h,the cell proliferation rate in the20μmol/L group was lower than that in the 5μmol/L group,the 10μmol/L group and the blank group(P<0.05).At24 h,48 h and 72 h,the apoptosis rate of the 20μmol/L group was higher than that of the 5μmol/L group,the 10μmol/L group and the blank group(P<0.05).The cell migration and invasiveness of 20μmol/L group were all lower than those of 5μmol/L group,10μmol/L group and blank group(P<0.05).The relative expression levels of Vimentin and Enhancer of zeste homolog 2(EZH2)in human cells in the 20μmol/L group were all lower than those in the 5μmol/L group,the 10μmol/L group,and the blank group.The relative expression levels of a-cat and e-cadherin were all higher than those of 5μmol/L group,10μmol/L group and blank group(P<0.05).Conclusion Zingiberenone can promote the apoptosis of HeLa cells and inhibit the proliferation and migration of HeLa cells.Invasiveness and capacity were concentration dependent.

关 键 词：花姜酮 HELA细胞 增殖 凋亡 迁移 

分 类 号：R285[医药卫生—中药学]