锦红片对急性胆源性感染大鼠TLR4及claudin-1表达的影响  

The Influence of Jinhong Tablets on Expressions of TLR4 and Claudin-1 in Rats with Acute Biliary Infection

作　　者：梁晓强[1] 余奎[1] 张静喆[1] LIANG Xiao-qiang;YU Kui;ZHANG Jing-zhe(Longhua Hospital Affiliated to Shanghai University of CM,Shanghai,China,200032)

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《河南中医》2020年第1期72-77,共6页Henan Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81173267)。

摘　　要：目的:观察锦红片对急性胆源性感染大鼠Toll样受体4(toll like receptor 4,TLR4)及claudin-1表达的影响。方法:将40只大鼠按随机数字表法分为对照组、模型组、锦红片组及庆大霉素组,每组各10只。模型组、锦红片组及庆大霉素组胰胆管内逆行注入大肠杆菌建立急性胆源性感染大鼠模型,对照组仅开腹静置10 min后关腹。造模后第1天,锦红片组给予锦红片溶液0.33 g·(kg·d)^-1灌胃,庆大霉素组给予庆大霉素溶液21.9 mg·(kg·d)^-1灌胃,对照组和模型组均在造模后第1天给予等量生理盐水灌胃。HE染色观察小肠大体形态学变化,ELASA法检测血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)及白细胞介素-6(interleukin-6,IL-6)含量。分别用real time-PCR和Western blot法检测小肠组织TLR4、claudin-1 mRNA及蛋白表达。结果:模型组大鼠小肠组织大体形态学存在病理改变,锦红片和庆大霉素均能改善以上病理改变。与对照组比较,模型组IL-1β、IL-6和TNF-α含量增加;与模型组比较,锦红片组IL-1β、IL-6和TNF-α含量降低,庆大霉素组IL-1β和TNF-α含量降低;与庆大霉素组比较,锦红片组IL-1β和TNF-α含量降低,差异均有统计学意义(P<0.05)。庆大霉素组与锦红片组IL-6含量比较,差异无统计学意义(P>0.05)。与对照组比较,模型组TLR4、claudin-1 mRNA表达增强;与模型组比较,锦红片组TLR4、claudin-1 mRNA表达降低,庆大霉素组claudin-1 mRNA表达降低,差异均有统计学意义(P<0.05)。庆大霉素组与锦红片组TLR4、claudin-1 mRNA表达比较,差异无统计学意义(P>0.05)。结论:锦红片治疗急性胆源性感染的作用机制可能为降低机体炎性因子水平,下调小肠TLR4、claudin-1 mRNA及蛋白表达,从而保护和修复肠黏膜屏障。Objective:To observe the clinical curative effect of Jinhong Tablets(JHT)on expressions of TLR4 and claudin-1 in rats with acute biliary infection.Methods:A total of 40 rats were randomly divided into the control group,the model group,the JHT group and the gentamicin group,with 10 rats in each group.The model group,the JHT group and the gentamicin group were injected in the opposite direction with escherichia coli to establish a rat model of acute biliary infection.In the control group,the abdomen was closed only after opening.On the first day after the establishment of the model,the JHT group was given JHT solution of 0.33 g·(kg·d)^-1 for gavage,while the gentamicin group was given gentamicin 21.9 mg·(kg·d)^-1 for gavage,the control group and the model group were given the same amount of normal saline gentamicin on the first day after the establishment of the model.He staining was used to observe the general morphological changes of small intestine,and ELASA method was used to detect the contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β) and interleukin-6(IL-6).Real time PCR and Western blot were used to detect TLR4,claudin-1 mRNA and protein expression respectively.Results:In the model group,there were pathological changes in the small intestine,which could be improved by both JHT and gentamicin.Compared with the control group,the contents of IL-1β,IL-6 and TNF-α in the model group increased.Compared with the model group,the contents of IL-1β,IL-6 and TNF-α decreased in the JHT group,while the contents of IL-1β and TNF-α decreased in the gentamicin group.Compared with the gentamicin group,the contents of IL-1β and TNF-α in the JHT group decreased significantly,and all the differences were statistically significant(P<0.05).There was no statistical difference in IL-6 between gentamicin group and Jinhong tablet group(P>0.05).In the model group,TLR4 and claudin-1 mRNA expression increased.Compared with the model group,the expressions of TLR4 and claudin-1 mRNA in the JHT group decrease

关 键 词：急性胆源性感染 锦红片 TLR4 CLAUDIN-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]