青蒿琥酯对哮喘小鼠嗜酸性粒细胞凋亡和Fas、Bcl-2基因蛋白表达的影响  

Effects of artesunate on eosinophil apoptosis and expressions of Fas and Bcl-2 proteins in asthmatic mice

作　　者：王瑞茵 林江涛[2] 王静茹 李春晓 WANG Ruiyin;LIN Jiangtao;WANG Jingru;LI Chunxiao(Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China;Department of Respiratory and Critical Care Medicine,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学研究生院,北京100029 [2]中日友好医院呼吸与危重症医学科,北京100029

出　　处：《南方医科大学学报》2020年第1期93-98,共6页Journal of Southern Medical University

基　　金：首都卫生发展科研专项重点攻关项目(2016-1-4061);北京市科委首都临床特色应用研究重点项目(2015-BKJ-001)

摘　　要：目的观察青蒿琥酯对哮喘小鼠嗜酸性粒细胞(EOS)凋亡以及相关基因Fas和Bcl-2蛋白表达的影响。方法 6~8周龄雌性BALB/c小鼠30只按随机数字表法分为对照组、哮喘组和青蒿琥酯组。除对照组外,其余各组均用卵白蛋白致敏并雾化激发制备哮喘小鼠模型。治疗组从造模第21天开始雾化激发前1 h腹腔注射给药。末次激发24 h后行肺组织染色,流式细胞术检测肺泡灌洗液(BALF)EOS的百分比和凋亡率,TUNEL法检测肺组织EOS凋亡情况,免疫组化法检测肺组织Fas和Bcl-2蛋白的表达。结果与哮喘组比较,青蒿琥酯组BALF中EOS的百分率下降(P<0.05),BALF和肺组织中EOS凋亡率升高(P<0.05)。肺组织中Fas蛋白的阳性面积和IOD升高(P<0.05),Bcl-2蛋白的阳性面积和IOD降低(P<0.05)。结论青蒿琥酯可调节Fas和Bcl-2的蛋白表达减少肺组织EOS浸润,促进EOS凋亡。Objective To observe the effects of artesunate on eosinophil(EOS) apoptosis and Fas and Bcl-2 protein expressions in asthmatic mice. Methods Thirty female BALB/c mice aged 6-8 weeks were randomly divided into control group, asthma group and artesunate group. Except for those in the control group, all the mice were sensitized with aerosolized ovalbumin to establish mouse models of asthma. In artesunate group, the rats were intraperitoneally injected with artesunate 1 h before ovalbumin inhalation from the 21 st day of modeling. The lung tissues were harvested for staining 24 h after the last challenge.Flow cytometry was used to analyze the percentage and apoptosis rate of EOS in the alveolar lavage fluid(BALF). The apoptosis of EOS in the lung tissue was detected with TUNEL method, and Fas and Bcl-2 protein expressions were detected using immunohistochemistry. Results Compared with those in asthma group, the artesunate-treated mice had significantly decreased percentage of EOS in the BALF(P<0.05) with increased apoptosis rate of EOS in the BALF and the lung tissue(P<0.05). The Fas-positive area and IOD of Fas protein in the lung tissue increased(P<0.05) while the Bcl-2-positive area and IOD of Bcl-2 protein decreased significantly in artesunate-treated mice as compared with the asthmatic mice(P<0.05).Conclusion Artesunate regulates the protein expressions of Fas and Bcl-2 to reduce EOS infiltration in the lung tissue and promote EOS apoptosis in asthmatic mice.

关 键 词：青蒿琥酯 哮喘 嗜酸性粒细胞 凋亡 FAS 

分 类 号：R285.5[医药卫生—中药学]