Epidermal growth factor improves intestinal morphology by stimulating proliferation and differentiation of enterocytes and mTOR signaling pathway in weaning piglets  

作　　者：Lixia Wang Fan Zhu Huansheng Yang Jianzhong Li Yali Li Xueqin Ding Xia Xiong Fengjie Ji Hanlin Zhou Yulong Yin 

机构地区：[1]Hunan International Joint Laboratory of Animal Intestinal Ecology and Health,Laboratory of Animal Nutrition and Human Health,College of Life Sciences,Hunan Normal University,Changsha 410081,China [2]Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central,Ministry of Agriculture,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Key Laboratory of Agroecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China [3]Academician Workstation of Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

出　　处：《Science China(Life Sciences)》2020年第2期259-268,共10页中国科学（生命科学英文版）

基　　金：supported by Key Programs of Frontier Scientific Research of the Chinese Academy of Sciences(QYZDYSSW-SMC008);Natural Science Foundation of Hunan Province(2017JJ1020);Young Elite Scientists Sponsorship Program by CAST(YESS20160086)。

摘　　要：Epidermal growth factor(EGF) has been shown to improve piglet intestinal morphology and epithelial recovery. In an attempt to further understand the mechanisms behind these improvements, this study tested the hypothesis that dietary EGF may affect intestinal morphology by stimulating the proliferation and differentiation of enterocytes in weaning piglets. In piglets receiving200 μg kg^-1 EGF, crypt depth and villus height increased(P<0.05). Adding 400 μg kg^-1 EGF increased villus height-to-crypt depth ratio(P<0.05), but reduced crypt depth(P<0.05). Dietary supplementation with 200 μg kg^-1 EGF significantly increased the number of Ki67-positive cells(P<0.01) and tended to increase the mRNA level of proliferating cell nuclear antigen(P<0.10).However, this supplementation decreased the expression level of intestinal fatty acid-binding protein(P<0.05). Piglets fed with400 μg kg^-1 EGF had an increased mRNA level of intestinal alkaline phosphatase(P<0.05). The phosphorylation of m TOR(mammalian target of rapamycin) was observed in the 200 μg kg^-1 EGF group. These results suggest that dietary supplementation with a low level of EGF improved piglet intestinal morphology through stimulating the proliferation and differentiation of enterocytes, and the mTOR signaling pathway may partly be involved in this process.Epidermal growth factor(EGF) has been shown to improve piglet intestinal morphology and epithelial recovery. In an attempt to further understand the mechanisms behind these improvements, this study tested the hypothesis that dietary EGF may affect intestinal morphology by stimulating the proliferation and differentiation of enterocytes in weaning piglets. In piglets receiving200 μg kg–1 EGF, crypt depth and villus height increased(P<0.05). Adding 400 μg kg–1 EGF increased villus height-to-crypt depth ratio(P<0.05), but reduced crypt depth(P<0.05). Dietary supplementation with 200 μg kg–1 EGF significantly increased the number of Ki67-positive cells(P<0.01) and tended to increase the mRNA level of proliferating cell nuclear antigen(P<0.10).However, this supplementation decreased the expression level of intestinal fatty acid-binding protein(P<0.05). Piglets fed with400 μg kg–1 EGF had an increased mRNA level of intestinal alkaline phosphatase(P<0.05). The phosphorylation of m TOR(mammalian target of rapamycin) was observed in the 200 μg kg–1 EGF group. These results suggest that dietary supplementation with a low level of EGF improved piglet intestinal morphology through stimulating the proliferation and differentiation of enterocytes, and the mTOR signaling pathway may partly be involved in this process.

关 键 词：EPIDERMAL growth factor ENTEROCYTE proliferation differentiation mTOR signaling WEANING PIGLETS 

分 类 号：S828[农业科学—畜牧学]