苗药了哥王“汗渍法”炮制前后抗小鼠免疫性炎症的“量-效-毒”关系研究  

Correlation Study of “Dose-effect-toxicity” of Miao Medicine Wikstroemia indica on Anti-immune Inflammation of Mice before and after Processed by “Sweat Soaking Method”

作　　者：郑传奇 冯果 李玮 周志容 许琴 李志平 易懂斌 李金徽 ZHENG Chuanqi;FENG Guo;LI Wei;ZHOU Zhirong;XU Qin;LI Zhiping;YI Dongbin;LI Jinhui(College of Pharmacy,Guizhou University of TCM,Guiyang 550025,China;National Engineering and Technology Research Center of Miao Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]国家苗药工程技术研究中心,贵阳550025

出　　处：《中国药房》2020年第6期661-665,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81760766);国家中医药管理局“中药炮制技术传承基地建设”项目(No.国中医药科技中药便函[2015]132);贵州省科技计划项目(No.黔科合基础[2017]1007);贵州省一流专业(中药学)建设项目(No.黔高教发[2017]158-02-32);贵州中医药大学科研项目(No.贵中医科院内[2016]40);贵州中医药大学博士启动基金项目(No.贵中医博士基金[2017]1)。

摘　　要：目的:探讨苗药了哥王炮制前后抗小鼠免疫性炎症的"量-效-毒"关系。方法:将小鼠分成空白组、模型组、了哥王生品醇提物1~6组、了哥王"汗渍法"炮制品醇提物1~6组(以下分别简称为"生品1~6组""炮制品1~6组",剂量均分别为0.13、0.20、0.26、0.52、1.04、2.6 g/kg)和阳性组(环磷酰胺,36.4 mg/kg),每组10只。除空白组外,其余各组小鼠均以1%2,4-二硝基氟苯-丙酮-麻油混合溶液复制迟发型超敏反应模型。造模后,空白组和模型组小鼠均灌胃等容1.0%羧甲基纤维素钠溶液,各给药组小鼠灌胃相应药液20 mL/kg,每日1次,连续5 d。末次给药后,记录各组小鼠耳肿胀度,并计算生品及炮制品的肿胀抑制率、半数有效剂量(ED50)及其95%置信区间(CI);称定小鼠心、肝、脾、肺、肾的质量,并计算各脏器指数;采用酶联免疫吸附测定法、改良化学氧化法分别检测小鼠血清中炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素10(IL-10)]和肝肾功能指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)、尿素氮(BUN)、血肌酐(CREA)]水平。结果:与空白组比较,模型组小鼠耳肿胀度显著升高(P<0.05);与模型组比较,了哥王生药及炮制品醇提物各剂量组小鼠耳肿胀度均显著降低(P<0.05),其肿胀抑制率均有随剂量增加而升高的趋势,两者抗小鼠迟发型超敏反应耳肿胀的ED50及95%CI分别为0.2396(0.1290,0.4452)g/kg、0.1473(0.0768,0.2827)g/kg。与空白组比较,模型组小鼠肝指数、血清TNF-α水平均显著升高,肺指数、血清IL-10水平均显著降低(P<0.05);与模型组比较,不同给药组小鼠肝指数(阳性组、生品3组和炮制品1~6组)、血清TNF-α水平(阳性组、生品1~3组及炮制品1~4组)均显著降低,肺指数(阳性组、生品3~6组及炮制品3~6组)、血清IL-10水平(生品1、2、4、5组以及炮制品2~6组)以及ALT、AST、BUN、CREA水平(生品4~6组)和TBIL水平(生品3~6组)均显著升高(POBJECTIVE:To investigate the"dose-effect-toxicity"correlation of Miao medicine Wikstroemia indica before and after processed on anti-immnue inflammation of mice. METHODS:Mice were divided into blank group,model group,ethanol extract of W. indica raw product groups 1-6,ethanol extract of W. indica processed product by"sweat soaking method"groups 1-6(hereinafter called"raw groups 1-6""processed groups 1-6"for short,drug dosage were 0.13,0.20,0.26,0.52,1.04,2.6 g/kg),positive group(cyclophosphamide,36.4 mg/kg),with 10 mice in each group. Except for blank group,other groups were given1% 2,4-dinitrofluorobenzene-acetone-sesame oil mixed solution to induce delayed type hypersensitivity model. After modeling,blank group and model group were given constant volume of 1.0% CMC-Na solution intragastrically,and administration groups were given relevant medicine 20 mL/kg intragastrically,once a day,for consecutive 5 d. After last medication,ear swelling degree of mice were recorded;the inhibition rate of swelling degree, half effective dose(ED50) and 95% confidence interval(CI) of raw and processed products were calculated;the weight of heart, liver, spleen, lung and kidney were measured and the indexes of organs were calculated;ELISA and modified chemical oxidation method were used to determine the serum levels of inflammatory factors(TNF-α,IL-10) and liver and renal function indexes(ALT, AST,TBIL,BUN,CREA). RESULTS:Compared with blank group,the degree of ear swelling in the model group was significantly increased(P<0.05). Compared with model group,ear swelling degree of mice were decreased significantly in different doses groups of ethanol extract of raw and processed products of W. indica(P<0.05). The inhibition rate of swelling increased with the increase of dose,ED50 and 95%CI of delayed hypersensitivity ear swelling were 0.239 6(0.129 0,0.445 2)g/kg and 0.147 3(0.0768,0.282 7)g/kg,respectively. Compared with blank group,liver index and serum TNF-α level of mice were increased significantly in model group,while lung in

关 键 词：了哥王 汗渍法 免疫性炎症 剂量 药效 毒性 

分 类 号：R285.5[医药卫生—中药学]