不同阶段骨关节炎滑膜组织中肾素、血管紧张素转换酶及血管紧张素受体1、2的表达研究  

Expressions of Renin,angiotensin converting enzyme,angiotensin receptor 1,and angiotensin receptor 2 in synovial tissue of osteoarthritis at different stages

作　　者：吴元刚[1] 曾羿[1] 李明阳 刘渊[1] 杨静[1] 沈彬[1] WU Yuangang;ZENG Yi;LI Mingyang;LIU Yuan;YANG Jing;SHEN Bin(Department of Orthopedics,West China Hospital,Sichuan University,Chengdu Sichuan,610041,P.R.China)

机构地区：[1]四川大学华西医院骨科,成都610041

出　　处：《中国修复重建外科杂志》2020年第3期362-366,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(81601936、81672219);四川省科技厅国际合作项目(2018HH0141);四川省卫健委重点项目(18ZD016)。

摘　　要：目的研究不同阶段骨关节炎滑膜组织中的肾素(Renin)、血管紧张素转换酶(angiotensin converting enzyme,ACE)、血管紧张素受体1(angiotensin receptor 1,AT1R)和AT2R的表达差异。方法以2018年1月-12月因创伤行膝上截肢术、骨关节炎行人工全膝关节置换术的患者作为研究对象。共32例患者符合选择标准纳入研究,根据Kellgren-Lawrence(K-L)X线分级标准分为正常滑膜组(A组,9例)、中度骨关节炎滑膜组(B组,11例,K-L 3级)及晚期骨关节炎滑膜组(C组,12例,K-L 4级)。采用实时荧光定量PCR及Western blot检测各组滑膜组织中Renin、ACE、AT1R、AT2R mRNA及蛋白相对表达量。结果B、C组Renin、ACE、AT1R mRNA及蛋白相对表达量明显高于A组,C组ACE、AT1R mRNA及蛋白相对表达量以及Renin蛋白相对表达量明显高于B组;而B、C组AT2R mRNA及蛋白相对表达量明显低于A组,C组低于B组;上述指标组间比较差异均有统计学意义(P<0.05)。结论随骨关节炎严重程度增加,关节滑膜组织中Renin、ACE、AT1R表达明显高于正常人群,AT2R表达量呈下降趋势,提示上述指标与骨关节炎发展相关。Objective To study the expressions of Renin,angiotensin converting enzyme(ACE),angiotensin receptor 1(AT1 R),and AT2 R in synovial tissue of osteoarthritis(OA)at different stages.Methods The patients who were treated with upper knee amputation because of trauma or total knee arthroplasty for OA between January 2018 and December 2018 were enrolled.Among them,32 patients who met the selection criteria were included in the study.According to the Kellgren-Lawrence(K-L)X-ray classification,they were allocated to normal synovial group(group A,n=9),moderate OA synovial group(group B,n=11,K-L level 3),and advanced OA synovial group(group C,n=12,K-L level 4).The relative expressions of Renin,ACE,AT1 R,and AT2 R mRNAs and proteins were detected by real-time fluorescence quantitative PCR(qRT-PCR)and Western blot.Results The relative expressions of Renin,ACE,and AT1 R mRNAs and proteins were significantly higher in group B and group C than in group A(P<0.05).The relative expressions of ACE and AT1 R mRNAs and proteins and Renin protein were significantly higher in group C than in group B(P<0.05).However,the relative expressions of AT2 R mRNA and protein were lower in group B and group C than in group A(P<0.05),and in group C than in group B(P<0.05).Conclusion The expressions of Renin,ACE,and AT1 R in synovial tissue of osteoarthritis significantly increase as the K-L level increased,and the expression of AT2 R decreases.Renin,ACE,AT1 R,and AT2 R have a certain degree of correlation with the development of OA.

关 键 词：骨关节炎 滑膜组织 肾素 血管紧张素转换酶 血管紧张素受体 

分 类 号：R684.3[医药卫生—骨科学]