水稻施硅对白背飞虱刺吸和寄主选择行为的影响  

Effects of silicon amendment to rice on piercing and host selection behaviors of the white-backed planthopper,Sogatella furcifera(Hemiptera:Delphacidae)

作　　者：贾路瑶 刘丹丹[1] 侯茂林[1] JIA Lu-Yao;LIU Dan-Dan;HOU Mao-Lin(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2020年第2期199-206,共8页Acta Entomologica Sinica

基　　金：国家重点研发专项(2018YFD0200306);国家自然科学基金项目(31371951)。

摘　　要：【目的】硅可增强植物对多种植食性昆虫的抗性。本研究旨在了解硅处理对水稻叶鞘硅化程度及其对白背飞虱Sogatella furcifera刺吸和寄主选择行为的影响,以明确施硅增强水稻对白背飞虱的抗性的部分机制。【方法】采用扫描电镜观察施硅水稻的叶鞘硅化程度;利用刺吸电位技术记录白背飞虱若虫刺吸行为;采用笼罩法测定白背飞虱雌成虫栖息和产卵选择性。【结果】与不施硅的对照相比,硅处理(0.16和0.32 g SiO2/kg土壤)增加了水稻叶鞘硅细胞的数量,延长了白背飞虱若虫刺吸行为中非刺探波和路径波的总时间,缩短了取食韧皮部汁液的时间。在选择性测定中,与对照组相比,白背飞虱雌成虫对0.16和0.32 g SiO2/kg土壤处理的水稻的栖息率分别降低48.0%和67.4%,在其上的产卵量分别降低34.8%和46.1%。【结论】施硅增加水稻对白背飞虱的排驱性,阻碍白背飞虱的刺吸行为,因此有助于增强水稻对白背飞虱的抗性。【Aim】Silicon(Si)amendment to plants can confer enhanced resistance to multiple herbivores.The study aims to understand the effects of Si amendment on silicification of rice leaf sheath and on piercing and host selection behaviors of the white-backed planthopper(WBPH),Sogatella furcifera,so as to identify Si-mediated rice resistance to WBPH.【Methods】Silicification of rice leaf sheath was observed using scanning electron microscope(SEM).The piercing behavior of WBPH nymphs was recorded with electrical penetration graph(EPG).The host selection and oviposition preference of female adults of WBPH were determined with cage test.【Results】Compared with the control plants without Si amendment,the rice plants amended with 0.16 or 0.32 g SiO2/kg soil were characterized by increased number of silica cells in the leaf sheaths.Si addition prolonged the non-probing event and pathway event and shortened the phloem ingestion event of WBPH nymphs.In the host selection tests,the settling preference of WBPH female adults for the rice plants amended with 0.16 and 0.32 g SiO2/kg soil decreased by 48.0%and 67.4%,respectively,than for the control plants,and the oviposition amount on these Si-amended plants decreased by 34.8%and 46.1%,respectively,than on the control plants.【Conclusion】Si amendment to rice plants enhances rice resistance to WBPH through increasing the antixenosis of rice to WBPH and impairing the piercing behavior of WBPH.

关 键 词：白背飞虱 硅 水稻 抗虫性 刺吸电位 刺吸行为 寄主选择 

分 类 号：Q968[生物学—昆虫学]