鼻腔滴入γ干扰素对变应性鼻炎大鼠外周血Th17/Treg细胞及相关细胞因子的影响  

Effects of intranasal infusion of IFN-γ on Th17/Treg cells in peripheral blood of rats with allergic rhinitis

作　　者：张美玲 孙卉 孙文凯 孟朝暾 邬信芳 高伟 李钦[5] ZHANG Meiling;SUN Hui;SUN Wenkai;MENG Zhaotun;WU Xinfang;GAO Wei;LI Qin(Weifang Clinical Medical College,Weifang Medical University,Weifang 261000,Shandong,China;School of Clinical Medicine,Binzhou Medical University,Binzhou 256600,Shandong,China;Medical College,Qingdao University,Qingdao 266000,Shandong,China;Graduate School,Shandong First Medical University,Taian 271000,Shandong,China;Department of Otorhinolaryngology&Head and Neck Surgery,Linyi People's Hospital,Linyi 276000,Shandong,China)

机构地区：[1]潍坊医学院临床医学院,山东潍坊261000 [2]滨州医学院临床医学院,山东滨州256600 [3]青岛大学医学部,山东青岛266000 [4]山东第一医科大学研究生院,山东泰安271000 [5]临沂市人民医院耳鼻咽喉头颈外科,山东临沂276000

出　　处：《山东大学学报（医学版）》2020年第1期13-19,共7页Journal of Shandong University:Health Sciences

基　　金：临沂市人民医院研究生培养基金(YJS2018047)。

摘　　要：目的探讨大鼠鼻腔滴入γ干扰素(IFN-γ)对变应性鼻炎(AR)大鼠外周血中辅助性T细胞17(Th17)、调节性T细胞(Treg)水平的影响。方法将40只Wistar雄性大鼠,按体质量编号、采用随机数字表法随机分为4组:对照组、致敏组、曲安奈德组、IFN-γ组,致敏阶段采用5级卵清蛋白(OVA)、氢氧化铝给予致敏组、曲安奈德组、IFN-γ组大鼠腹腔注射致敏以及OVA滴鼻激发造模,对照组腹腔注射以生理盐水替代,滴鼻以超纯水替代;给药阶段对照组不予处理,致敏组、曲安奈德组、IFN-γ组每只每侧鼻孔分别给予50μL PBS、3.5μg曲安奈德、1μg IFN-γ滴鼻,于末次给药结束24 h内行流式细胞术检测外周血Th17细胞、Treg细胞所占比例,1周内行定量酶联免疫吸附法(ELISA)检测外周血中白介素-17A、转化生长因子(TGF)-β1的浓度,并行苏木精-伊红(HE)染色,观察黏膜情况。结果与对照组[(0.2134±0.1495)%]相比,致敏组[(4.9757±1.5039)%]、曲安奈德组[(1.1693±0.3861)%]、IFN-γ组[(1.5320±0.5265)%]的Th17细胞比例都不同程度升高(F=51.532,P<0.05),除曲安奈德组、IFN-γ组间比较无统计学意义(P>0.05)外,其余各组间比较有统计学意义(P<0.05);与对照组[(22.9393±2.0995)%]相比,致敏组[(8.2232±2.1888)%]、曲安奈德组[(14.0875±1.3696)%]、IFN-γ组[(16.6143±1.62170)%]的Treg细胞比例不同程度降低(F=97.107,P<0.05),除曲安奈德组、IFN-γ组间比较无统计学意义(P>0.05)外,其余各组间比较有统计学意义(P<0.0083);与对照组[(5.0769±1.4356)pg/mL]相比,致敏组[(34.9005±11.6708)pg/mL]、曲安奈德组[(9.7338±1.8289)pg/mL]、IFN-γ组[(10.5246±2.1180)pg/mL]的IL-17A浓度升高(F=27.173,P<0.05),除曲安奈德组、IFN-γ组间比较无统计学意义(P>0.05)外,其余各组间比较有统计学意义(P<0.05);与对照组[(431.8816±67.3262)pg/mL]相比,致敏组[(173.8862±41.7082)pg/mL]、曲安奈德组[(246.0533±13.8412)pg/mL]、IFN-γ组[(290.0127±22.8856)pg/mL]的TGF-�Objective To investigate the effects of interferon gamma(IFN-γ) on T helper cell 17(Th17) and regulatory T cell(Treg)(Th17/Treg) in peripheral blood of rats with allergic rhinitis(AR). Methods A total of 40 Wistar male rats were randomly divided into 4 groups: control group, sensitized group, triamcinolone acetonide group, and IFN-γ group. The control group was injected with saline intraperitoneally, and had ultra-pure water nasal drops. The other 3 groups were injected with ovalbumin(OVA) and aluminium hydroxide and had OVA nasal instillation to establish AR models. After that, the sensitized group was treated with 50 μL phosphate buffer(PBS), triamcinolone acetonide group was treated with 3.5 μg triamcinolone acetonide, and IFN-γ group was treated with 1 μg IFN-γ nasal drops on either nostril. The proportions of Th17 cells and Treg cells in peripheral blood were measured with flow cytometry within 24 hours after the last administration, and levels of interleukin-17 A(IL-17 A) and transforming growth factor(TGF-β1) were detected with quantitative enzyme-linked immunosorbent assay(ELISA) within 1 week. The nasal mucosa was observed with hematoxylin-eosin(HE) staining. Results Compared with control group [(0.213 4±0.149 5)%], the percentage of Th17 cells in sensitized group [(4.975 7±1.503 9) %], triamcinolone acetonide group [(1.169 3±0.386 1) %] and IFN-γ group [(1.532 0±0.526 5) %] increased in varying degrees(F=51.532, P<0.05), with no statistical difference between the triamcinolone acetonide group and IFN-γ group(P>0.05), but with differences among the other groups(P<0.05). Compared with control group [(22.939 3±2.099 5)%], the proportion of Treg cells in sensitized group [(8.223 2±2.1888)%], triamcinolone acetonide group [(14.087 5±1.369 6) %] and IFN-γ group [(16.614 3±1.621 7) %] decreased in varying degrees(F=97.107, P<0.05), with no difference between the triamcinolone acetonide group and IFN-γ group(P>0.05), but with differences among the other groups(P<0.008 3). Compared with contr

关 键 词：动物模型 变应性鼻炎 Γ干扰素 Th17细胞 Treg细胞 白介素17A 转化生长因子 WISTAR大鼠 

分 类 号：R765[医药卫生—耳鼻咽喉科]